Kisker - Products for Biotechnology

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Electropheresis

Dry block heat bath

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Rotator & Vortex Mixer

Nylon Wool & PAP PEN

PCR & DNA Measurement

Microparticles

0,00 €

Nylon Wool & PAP PEN

PAP PEN

PAP PEN is designed to provide a water repellant barrier when a circle is drawn around a specimen. The barrier of the PAP PEN retains antisera within the defined area and ensures, that only the amount of antobody needed for sufficient reaction is used. The PAP PEN stops spreading, reduces waste and divides slides into discret areas for immunostaining, histology, cytology, cell culture, virology, microbiology. The PAP PEN is effective for immunostaining, PAP method, ABC method, ASD method, enzyme and frozen section methods.
The staining is unsoluble in aceton and alcohol and can be removed if desired by xylene after the staining procedure.

Cat. #	Description	Euro	Units
MKP-1	Pap Pen Immunostaining Pen	46,00
MKP-2	Pap Pen Immunostaining Pen, extra thick	59,90

Neubauer Improved / Disposable Hemocytometer

The Neubauer Improved - C-Chip is a disposable plastic one-time-use hemocytometer used for manual cell-counting. It consists of two surface patterned chambers with two parts for sample injection. The Hemotocytometer is made from plastic with glass-characteristics for standarized countings.
The handling is easy, 10 µl of cell suspension will be injected on the chip and counted. The grid pattern is exactly the same as the "Neubauer Improved" chamber. It consits of nine large squares of each 1 x 1 mm with 0,1 mm depth. Each square has a total volume of 0,3 cmm or 10E-4 ccm. The central square is divided into 25 small squares with triple lines and four corner squares are divided into 16 smaller squares

Methology 1. General methods with C-Chip: mix the samples well, load 10 µl of sample into the sample injection area so that the chamber is filled by capillary action, count the cells under the microscope
2. Mammalian-Cell Counting: treat the cell samples with trypsin-EDTA, carefully remove the supernatant with a pipett tip and avoid to disturb the pellet, add an appropriate volume of growth medium or PBS to dilute to a final concentration of 5 x 10E3 cells / ml to 5 x 10E6 cells/ ml
3. Erythrocyte counting ( 1:200 dilution): dilute blood using accepted laboratory methodes, load 10 µl deluted sample into the injection area, count the erythrocytes in the five small squares ( four small corner-squares and one small middle square) of the large center square
Leukocyte counting (1:20 dilution): dilute blood using accepted laboratory methodes, load 10 µl deluted sample into the injection area, count the leucocytes in the four large corner squares.

cat.#	Description	Pack size	Euro	Units
M-NZ	C-Chip	50 Chips	78,00

Combed, Scrubbed Nylon Wool Fiber, Type R Separation of T-cell from B-cell lymphocytes with Nylon Wool Fiber

Mononuclear leukocyte concentrates from freshly drawn blood can be separated into T-cell and B-cell enriched fractions using "Nylon Wool " Mononuclear lymphoid cells may be preseparated from the polymorphonuclear (PMN) leukocytes and contaminating erythrocytes by centrifugation on an isopynknotic solution of IsoPrep (specific gravity 1.077gm/liter). If an isopynknotic solution is not used, the polymorphonuclear (PMN) leucocytes will adhere tightly to the nylon wool, while any erythrocytes will be eluted with the nonadherent cell fraction. Prior to the fractionation procedure, the mononuclear cells are washed once in Hank`s Balanced Salt Solution, counted and resuspended in Hank`s Balanced Salt Solution supplemented with 10% fetal calf serum (FCS) or in other cell growth media like RPMI 1640 with 10% FCS, Earl`s Saline with 10% FCS or Dulbecco`s PBS with 5% FCS.

Our Nylon wool works well with e.g. human, mouse and rat cells. Please see also: current protocols in Immunology (1992) 3.2.1-3.2.4, contributed by K.S.Hathcock, T-Cell Enrichment by nonadherence to Nylon, John Wiley & Sons, Inc.
Cell Separation Procedure

1) T-cell enriched fraction
Pack tightly 0,5gram of sterile Nylon wool to a 10ml sterile syringe. Wash and equilibrate the Nylon Wool with selected media HBSS or RPMI 1640 with 10% FCS.
1-2 x 108 mononuclear

Cat. #	Description	Pack size	Euro	Units
MKN-10	Nylon Wool Fiber	10g	38,00
MKN-50	Nylon Wool Fiber	50g	119,00
MKN-100	Nylon Wool Fiber	100g	209,00

Slide Boxes colored, for 100 Slides

The slide boxes have cork liming on the bottom, to protect the slides. On the inside cover of the box is a corresponding inventory slide sheet. The boxes can be stored securely on top of each other and are closed with a rust resistant nickel plated clasp ande hinge pin.Dimensions are 222 x 171 x 33mm ( LxWxH )

Optional we offer stainless steeel freezer racks to store the slide boxes in upright freezers for longtime storage of temperature sensitive samples. You find infos and prices in the chapter CRYO PRODUCTS.

cat.#	Color	Pack size	Euro	Units
R100-B	blue	1	4,95
R100-G	green	1	4,95
R100-Y	yellow	1	4,95
R100-R	red	1	4,95
R100-W	white	1	4,95
R100-GRA	grey	1	4,95
R100-SCH	black	1	4,95

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