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Polystyrene- Coated- Flow Cytometry- PMMA

 
Polystyrene microparticles
These monodisperse polystyrene microparticles, also called Latex-microspheres, are used in a wide range of immunodiagnostic assays, as size-standards for calibration of equipment, in cellbiology applications etc. The physical adsorption of the polystyrene particles is used to bind ligands to the surface of the particles.

Some polystyrene particles can be offered with special modifications e.g. acrylate, epoxy, NI-NTA, collagen or Au, please ask for quotation.
Cat. #size/quantity/concentrationchemistryEuroAnzahl
PPs-0.0150,015µ/ 10ml / 1,0%plain190,00
PPs-0.0250,025µ/ 10ml / 1,0%plain190,00
PPs-0.050,05µ/ 15ml / 2,5%plain60,80
PPs-0.10,1µ/ 15ml / 2,5%plain50,10
PPs-0.20,2µ/ 15ml / 2,5%plain50,10
PPs-0.40,4µ/ 15ml / 2,5%plain50,10
PPs-0.60,6µ/ 15ml / 2,5%plain50,10
PPs-0.80,8µ/ 15ml / 2,5%plain55,75
PPs-1.01,0µ/ 15ml / 2,5%plain60,80
PPs-1.21,0-1,4µ/ 10ml / 5%plain133,00
PPs-1.2-B1,0-1,4µ/ 100ml / 5%plain885,00
PPs-1.71,5-1,9µ/ 10ml / 5%plain133,00
PPs-1.7-B1,5-1,9µ/ 100ml / 5%plain885,00
PPs-2.02,0µ/ 10ml / 2,5%plain79,80
PPs-2.22,0-2,4µ/ 10ml / 5%plain146,00
PPs-2.2-B2,0-2,4µ/ 100ml / 5%plain850,00
PPs-2.72,5-2,9µ/ 10ml / 5%plain149,80
PPs-2.7-B2,5-2,9µ/ 100ml / 5%plain879,00
PPs-3.03,0µ/ 10ml / 2,5%plain95,80
PPs-3.23,0-3,4µ/ 10ml / 5%plain156,00
156,003,0-3,4µ/ 100ml / 5%plain998,00
PPs-3.73,5-3,9µ/ 10ml / 5%plain156,00
PPs-3.7-B3,5-3,9µ/ 100ml / 5%plain998,00
PPs-4.04,0µ/ 5ml / 2,5%plain76,20
PPs-4.24,0-4,4µ/ 10ml / 5%plain169,80
PPs-4.2-B4,0-4,4µ/ 100ml / 5%plain1.098,00
PPs-4.74,5-4,9µ/ 10ml / 5%plain169,80
PPs-4.7-B4,5-4,9µ/ 100ml / 5%plain1.098,00
PPs-5.05,0µ/ 5ml / 2,5%plain76,20
PPs-5.55,0-5,9µ/ 10ml / 5%plain172,90
PPs-5.5-B5,0-5,9µ/ 100ml / 5%plain1.195,00
PPs-6.06,0µ/ 5ml / 2,5%plain76,20
PPs-7.07,0µ/ 5ml / 2,5%plain76,20
PPs-8.08,0µ/ 5ml / 2,5%8,0µ/ 5ml / 2,5%76,20
PPs-10.010,0µ/ 5ml / 2,5%plain95,80
PPs-12.012,0µ/ 5ml / 2,5%plain95,80
PPs-15.015,0µ/ 5ml 2,5%plain95,80
PPs-20.020,0µ/ 10ml 2,5%plain119,00
PPs-25.025,0µ/ 10ml 2,5%plain119,00
PPs-30.030,0µ/ 5ml / 2,5%plain119,00
PPs-65.065,0µ/ 10ml / 2,5%plain119,00
 
Polystyrene microparticles , carboxylated
Monodisperse polystyrene microparticles, which contain carboxyl groups at the surface. They are used for passive adsorption or covalent binding of proteins.
Cat. #size/quantity/concentrationchemistryEuroAnzahl
PPs-0.015COOH0,015µ/ 10ml / 1,0%COOH219,00
PPs-0.025COOH0,025µ/ 10ml / 1,0%COOH219,00
PPs-0.05COOH0,05µ/ 15ml / 1,0%COOH219,00
PPs-0.1COOH0,1µ/ 15ml / 5,0%COOH145,00
PPs-0.2COOH0,2µ/ 15ml / 5,0%COOH145,00
PPs-0.4COOH0,4µ/ 15ml / 5,0%COOH145,00
PPs-0.5COOH0,5µ/ 10ml / 5,0%COOH135,00
PPs-0.5COOH-B0,5µ/ 100ml / 5,0%COOH875,00
PPs-0.6COOH0,6µ/ 15ml / 5,0%COOH145,00
PPs-0.8COOH0,8µ/ 15ml / 5,0%COOH145,00
PPs-1.0COOH1,0µ/ 10ml / 5,0%COOH150,00
PPs-1.2COOH1,0-1.4µ/ 10ml / 5,0%COOH146,00
PPs-1.2COOH-B1,0-1.4µ/ 100ml / 5,0%COOH998,00
PPs-1.7COOH1,5-1.9µ/ 10ml / 5,0%COOH146,00
PPs-1.7COOH-B1,5-1.9µ/ 100ml / 5,0%COOH998,00
PPs-2.0COOH2,0µ/ 10ml / 5,0%COOH150,00
PPs-2.2COOH2,0-2.4µ/ 10ml / 5,0%COOH157,00
PPs-2.2COOH-B2,0-2.4µ/ 100ml / 5,0%COOH1.078,00
PPs-2.7COOH2,5-2.9µ/ 10ml / 5,0%COOH165,00
PPs-2.7COOH-B2,5-2.9µ/ 100ml / 5,0%COOH1.522,00
PPs-3.0COOH3,0µ/ 10ml / 5,0%COOH155,00
PPs-3.2COOH3,0-3.4µ/ 10ml / 5,0%COOH169,80
PPs-3.2COOH-B3,0-3.4µ/ 100ml / 5,0%COOH1.155,00
PPs-3.7COOH3,5-3.9µ/ 10ml / 5,0%COOH169,80
PPs-3.7COOH-B3,5-3.9µ/ 100ml / 5,0%COOH1.155,00
PPs-4.0COOH4,0µ/ 10ml / 5,0%COOH155,00
PPs-4.2COOH4,0-4.4µ/ 10ml / 5,0%COOH198,00
PPs-4.2COOH-B4,0-3.4µ/ 100ml / 5,0%COOH1.385,00
PPs-4.7COOH3,5-3.9µ/ 10ml / 5,0%COOH198,00
PPs-4.7COOH-BPPs-4.7COOH-BCOOH1.385,00
PPs-5.5COOH5,0-5.9µ/ 10ml / 5,0%COOH210,00
PPs-5.5COOH-B5,0-5.9µ/ 100ml / 5,0%COOH1.398,00
PPs-6.0COOH6,0µ/ 10ml / 5,0%COOH185,00
PPs-8.0COOH8,0µ/ 10ml / 5,0%COOH185,00
 
Polystyrene microparticles , amino modified
Monodisperse polystyrene microparticles, which contain amino groups at the surface. They are used for covalent binding of proteins with the glutaraldehyde methode.
Cat. #size/quantity/concentrationchemistryEuroAnzahl
PPs-0.2NH20,2µ/ 10ml / 2,5%NH2129,00
PPs-0.2NH2-B0,2µ/ 100ml / 2,5%NH2885,00
PPs-0.5NH20,5µ/ 10ml / 5,0%NH2129,00
PPs-0.8NH20,8µ/ 10ml / 5,0%NH2129,00
PPs-0.8NH2-B0,8µ/ 100ml / 5,0%NH2885,00
PPs-1.0NH21,0µ/ 10ml / 5,0%NH2136,00
PPs-1.0NH2-B1,0µ/ 100ml / 5,0%NH2998,00
PPs-2.0NH22,0µ/ 10ml / 5,0%NH2136,00
PPs-2.0NH2-B2,0µ/ 100ml / 5,0%NH21.078,00
PPs-2.5NH22,5µ/ 10ml / 5,0%NH2145,00
PPs-2.5NH2-B2,5µ/ 100ml / 5,0%NH21.150,00
PPs-3.0NH23,0µ/ 10ml / 5,0%NH2149,00
PPs-3.0NH2-B3,0µ/ 100ml / 5,0%NH21.150,00
PPs-3.5NH23,5µ/ 10ml / 5,0%NH2149,00
PPs-3.5NH2-B3,5µ/ 100ml / 5,0%NH21.150,00
PPs-7.0NH26,0-7,9µ/ 10ml / 5,0%NH2195,00
PPs-7.0NH2-B6,0-7,9µ/ 100ml / 5,0%NH21.495,00
PPs-10.0NH210,0µ/ 10ml / 5,0%NH2209,00
PPs-12.0NH212,0µ/ 10ml / 5,0%NH2209,00
 
Powdered monodisperse Polystyrene size-particle-standards
These particles have CV under 5% and can be used, to check ot calibrate particel-size-systems.
Cat. #size/quantitychemistryEuroAnzahl
PPs-6.0P6,0µ/ 1000mgplain269,00
PPs-8.0P8,0µ/ 1000mgplain269,00
PPs-12.0P12,0µ/ 1000mgplain269,00
PPs-20.0P20,0µ/ 1000mgplain269,00
 
Polystyrene non-fluorescent colored microparticles
These polystyrene microparticles are prepared by polymerizing non-fluorescent dye in the styrene. The particles are free of solvent commonly found when particles are prepared by staining with a solution of dye in inorganic solvent. The intensely colored particles enhance the visual detection in assays such as latex agglutination, dipstick and membrane based assays.

The polystyrene particles are offered in 5% aqueous suspension, exept PPsB-7.0, which is 1%
Cat. #sizeQuantity/Conc.chemistryEuroAnzahl
PPsB-0.50,4-0,06µ10ml/5%w/vplain119,00
PPsB-0.5-B0,4-0,06µ100ml/5%w/vplain775,00
PPsB-7.06,0-7.9µ5ml/1%w/vplain135,00
PPsB-0.04COOH0,03-0.06µ10ml/5%w/vCOOH135,00
PPsB-0.1COOH0,1-0.19µ10ml/5%w/vCOOH135,00
PPsB-0.1COOH-B0,1-0.19µ100ml/5%w/vCOOH885,00
PPsB-0.2COOH0,2-0.29µ10ml/5%w/vCOOH135,00
PPsB-0.2COOH-B0,2-0.29µ100ml/5%w/vCOOH885,00
PPsB-0.3COOH0,3-0.39µ10ml/5%w/vCOOH135,00
PPsB-0.3COOH-B0,3-0.39µ100ml/5%w/vCOOH885,00
PPsB-0.5COOH0,4-0.6µ10ml/5%w/vCOOH135,00
PPsB-0.5COOH-B0,4-0.6µ100ml/5%w/vCOOH885,00
 
Coated Polystyrene Particles
The coated microparticles are prepared either by passive adsorption or covalent coupling depending upon the intended applications. The avidin and biotin coated particles are prepared by covalent coupling. Even through the 0,7 - 0,9µ Goat anti-mouse IgG coated polystyrene particles are prepared by using passive adsorption, the coated particles are stable for several years under proper storage conditions.
Some typical data for Avidin polystyrene particles (0,8µ) are:
Covalently coated with egg white avidin, avidin content 14 µg/mg solid, 0.212 nmole/mg solid
Binding capacity to Biotin-Fluorescein 0,46 nmole/mg solid

Antibody coated polystyrene particles (0,8µ):
Antibody contents 14µg/mg solid, 0,2µg/cm2, 1,5 x 10E4 IgG/particle, binding capacity to IgG-FITC 4µg/mg
Cat. #DescriptionSizeµ/ unit% w/vEuroAnzahl
PC-Fc-0.8Goat Anti-mouse IgG polystyrene,affin.pur., Fc specific0,7-0,9/20ml0,25229,00
PC-HL-0.8Goat Anti-mouse IgG polystyrene,affin.pur., H & L0,7-0,9/20ml0,25229,00
PC-CAHL-0.8Goat Anti-mouse IgG poly.,affin.pur./cross absorbed,H&L0,7-0,9/20ml0,25249,00
PC-CAR-0.8Goat Anti-mouse IgG poly.,affin.pur./cross absorbed,H&L0,7-0,9/20ml0,25249,00
PC-Fc-3.0Goat Anti-mouse IgG (Fc) polystyrene3,0-3,9/ 5ml0,5273,10
PC-Fc-6.0Goat Anti-mouse IgG (Fc) polystyrene6,0-8,0/ 5ml0,5273,10
PCAH-HL-0.8Goat Anti-Human IgG (H&L) polystyrene0,7-0,9/ 5ml0,25149,00
PCAH-HL-3.0Goat Anti-Human IgG (H&L) polystyrene3,0-3,9/ 5ml0,5247,00
PCAH-HL-7.0Goat Anti-Human IgG (H&L) polystyrene6,0-8,0/ 5ml0,5279,00
PC-A-0.7Avidine-polystyrene0,7-0,9/10ml5319,00
PC-A-1.0Avidine-polystyrene1,0-1,4/10ml5319,00
PC-A-3.0Avidine-polystyrene3,0-3,9/5ml0,5239,00
PC-A-6.0Avidine-polystyrene6,0-8,0/ 5ml0,5239,00
PC-S-0.3Streptavidine-polystyrene0,3-0,39/10ml1249,00
PC-S-0.4Streptavidine-polystyrene0,4-0,6/10ml1249,00
PC-S-0.8Streptavidine-polystyrene0,7-0,9/10ml1249,00
PC-S-1.5Streptavidine-polystyrene1,5-1,9/ 5ml1249,00
PC-S-2.0Streptavidine-polystyrene2,0-2,9/ 5ml0,5258,60
PC-S-3.0Streptavidine-polystyrene3,0-3,9/ 5ml0,5258,60
PC-S-4.0Streptavidine-polystyrene4,0-4,9/ 5ml0,5258,60
PC-S-5.0Streptavidine-polystyrene5,0-5,9/ 5ml0,5258,60
PC-S-6.0Streptavidine-polystyrene6,0-8,0/ 5ml0,5258,60
PC-S-10.0Streptavidine-polystyrene10,0-13,9/ 4ml0,5299,60
PC-S-15.0Streptavidine-polystyrene13,0-17,9/ 4ml0,5347,60
PC-S-90.0Streptavidine-polystyrene80,0-95,0/ 4ml1415,00
PC-S-20.0Streptavidine-polystyrene18,0-24,0/ 4ml0,5369,60
PC-B-0.8Biotin-polystyrene0,7-0,9/ 10ml1249,60
PC-B-3.0Biotin-polystyrene3,0-3,9/ 5ml0,5278,60
PC-B-6.0Biotin-polystyrene6,0-8,6/ 5ml1315,85
PC-BX-6.0Biotin-polystyrene, 0,5% DVB X-linked6,0-8,0/ 5ml1279,00
PC-BX-10.0Biotin-polystyrene, 0,5% DVB X-linked8,0-13,0/ 5ml0,5360,00
PC-BX-15.0Biotin-polystyrene, 0,5% DVB X-linked13,0-18,0/ 5ml0,5420,00
PC-PA-0.8Protein A-polystyrene0,7-0,9/5ml1170,00
PC-PA-2.0Protein A-polystyrene2,0-2,9/5ml0,5170,00
PC-PA-4.0Protein A-polystyrene4,0-4,5/5ml0,5170,00
PC-PA-7.0Protein A-polystyrene6,0-8,0/5ml0,5269,00
PC-PG-0.5Protein G-polystyrene0,4-0,6/5ml1199,00
PC-PG-0.8Protein G-polystyrene0,7-0,9/5ml1199,00
PC-PG-2.0Protein G-polystyrene2,0-2,9/5ml0,5199,00
PC-PG-3.0Protein G-polystyrene3,0-3,5/5ml0,5199,00
PC-PG-4.0Protein G-polystyrene4,0-4,9/5ml0,5199,00
PC-PG-7.0Protein G-polystyrene6,0-8,0/5ml0,5269,00
 
Coated Fluorescent Polystyrene Particles
Cat. #DescriptionSizeµ/ unit% w/vEuroAnzahl
PC-AFY-0.5Avidin yellow0,4-0,6/ 5ml0,1159,00
PC-AFP-0.5Avidin pink0,4-0,6/ 5ml0,1159,00
PC-AFU-0.5Avidin purple0,4-0,6/ 5ml0,1159,00
PC-AFY-0.8Avidin yellow0,7-0,9/ 5ml0,1159,00
PC-AFN-0.8Avidin nile red0,7-0,9/ 5ml0,1159,00
PC-AFU-0.8Avidin purple0,7-0,9/ 5ml0,1159,00
PC-AFY-2.0Avidin yellow1,7-2,2/ 5ml0,1199,00
PC-AFP-2.0Avidin pink1,7-2,2/ 5ml0,1199,00
PC-AFSB-0.8Avidin Sky Blue0,7-0,9/ 5ml0,1159,00
PC-AFITC-1.7Avidin FITC1,7-2,2/ 5ml0,1199,00
PC-AFP-1.7Avidin pink1,7-2,2/ 5ml0,1199,00
PC-AM-FY-0.8Anti mouse yellow IgG H&L0,7-0,9/ 5ml0,1159,00
PC-AM-FP-0.8Anti mouse pink IgG H&L0,7-0,9/ 5ml0,1159,00
PC-AM-FU-0.8Anti mouse purple IgG H&L0,7-0,9/ 5ml0,1159,00
PC-AM-FN-0.8Anti mouse nile red IgG H&L0,7-0,9/ 5ml0,1159,00
PC-AM-FY-1.7Anti mouse yellow IgG H&L1,7-2,2/ 5ml0,1159,00
PC-AM-FP-1.7Anti mouse pink IgG H&L1,7-2,2/ 5ml0,1159,00
PC-AM-FU-1.7Anti mouse purple IgG H&L1,7-2,2/ 5ml0,1159,00
PC-AM-FN-1.7Anti mouse nile red IgG H&L1,7-2,2/ 5ml0,1159,00
PC-AM-FcFY-2.5Anti mouse yellow IgG (FC)2,5-4,5/ 5ml0,1219,00
PC-AM-FcFP-2.5Anti mouse pink IgG (Fc)2,5-4,5/ 5ml0,1219,00
PC-AM-FcFN-2.5Anti mouse niole red IgG (Fc)2,5-4,5/ 5ml0,1219,00
PC-BFY-0.5Biotin yellow0,4-0,6/ 5ml0,1159,00
PC-BFN-0.5Biotin nile red0,4-0,6/ 5ml0,1159,00
PC-BFP-0.8Biotin pink0,7-0,9/ 5ml0,1159,00
PC-BFP-2.0Biotin pink1,7-2,2/ 5ml0,1199,00
PC-BFB-3.0Biotin blue3,0-3,9/ 5ml0,1199,00
PC-BFP-5.0Biotin pink5,0-5,9/ 5ml0,1199,00
PC-BFY-7.0Biotin yellow7,0-7,9/ 5ml0,1244,00
PC-BFN-7.0Biotin nile red7,0-7,9/ 5ml0,1244,00
PC-BFB-7.0Biotin blue7,0-7,9/ 5ml0,1244,00
PC-PAFY-0.5Protein A yellow0,4-0,6/ 5ml0,1199,00
PC-PAFN-0.5Protein A red0,4-0,6/ 5ml0,1199,00
PC-PAFP-0.5Protein A pink0,4-0,6/ 5ml0,1199,00
Available Data Sheets on Request:
1. Particle coating procedures
2. Determination of Antibody binding to Particles
 
Fluorescent Polystyrene Particles
The Fluorescent Particles are prepared by either staining the Polystyrene with the fluorophore or by polymerizing the fluorophore in styrene in the presence of polystyrene particles. A wide varity of fluorescent particles can be prepared ranging in size, type of fluorophore, fluorecent intensity and surface functional groups. Most of the used fluorophores are waterinsoluble and therefore very stable. These fluorophores incorporated into the particles do not leach and their color and fluorescence remains stable for a long time under proper storage conditions.

The exicitation and emmission data of the fluorecent particles in aqueous solution are shown in the figure. The particles are monodisperse, the stated diameters are nominal, the actual diameter is stated on each bottle.

Fluorescent Data:
Ex/Em max (nm)Particle typeEX(nm) filterEM(nm) filterCompatible fluorophores
390/450Light yellow360 – 390450Hoechst 33342, 33258
470/480Yellow470 – 490520FITC
525/560Nile red488 – 540560R-phycoerythrin, Propidium Iodide
570/590Pink550 – 570580Propidium iodide
590-620Purple590620Texas red
620/650Nile blue632 – 635650Allophycocyanin, Cy5
680/710Sky blue632 - 670700Cy5, Cy7, IR123
Multi-Fluo360 – 635400 – 680Contains multiple fluorophores
 
FITC Polystyrene Particles for Flow cytometry
The FITC labeled polystyrene particles can be used as calibration particles for flow cytometry. They can also be used to cross calibrate different flow cytometers so that data can be normalized.

Please note, that the size ranges stated below are nominal, the actual size of these monodisperse particles is stated on each bottle.
Cat. #Type & ColorSize in µ% w/vpacksizeEuroAnzahl
PFITC-0.8FITC0,7 –0,90,12ml175,00
PFITC-2.0FITC2,0 - 2,90,12ml175,00
PFITC-3.0FITC3,0 – 3,90,12ml187,00
PFITC-5.0FITC5,0 – 5,90,12ml198,00
PFITC-7.0FITC7,0 – 7,90,12ml214,00
PFITC-8.0FITC8,0 – 8,90,12ml214,00
 
Flow Cytometry Calibration Particles
 
Easy Calibration Fluorescent Particles
The Easy Calibartion Fluorescent particles are usewd to detect changes within the flow cytometer such as contaminats or pH changes.
The beads consits of a mixture of particles with intensities calibrated in terms of "Molecules of Equivalent Fluorochrome" (MEF) units.They are offered with three different fluorochromes including FITC,PE and PE-Cy-5.
Since each Easy Calibration Fluorescent Particle population is assigned a MEF unit, linearity calibrations and quantitative determinations can be performed. A linearity calibrations is performed by graphing each population´s channel number against it assigned MEF value. As a result, a regression line is created. This line can be used to track the performancy history of the instrument.
Cat. #Easy Calibration particlesSizeµ/ unitBeadsEuroAnzahl
ECFP-F1-5KFITC 5 peaks3,0-3,4/ 5x1ml2x10E6 / ml257,00
ECFP-F2-5KPE 5 peaks3,0-3,4/ 5x1ml2x10E6 / ml257,00
ECFP-F4-5KPE-Cy-5 5 peaks3,0-3,4/ 5x1ml2x10E6 / ml257,00
 
Ultra Rainbow Calibration Particles
A new type of Calibration Particles is now available for performance tracking of flow cytometers (e.g. BD FC-500, Partec, LSR Ia & II, Canto) with fluorescet channels in the Far Red. They are available in 3.8 µ or 5.05 µ. The Ultra Rainbow Calibration Particles have improved resolution in the PE-Cy7, APC and APC-Cy7 channels. These particles are supplied as a kit with 3 ml of blank particles and 2 ml of fluorescent particles.
The histograms showing individual peaks of cat.# P-URCP-3.8
Cat. #Rainbow Calibration particlesSizeµ/ unitBeadsEuroAnzahl
P-URCP-3.86 peaks3,5-3,9/ 2 ml10E7 / ml265,00
P-URCP-5.06 peaks5,0-5,9/ 2 ml10E7 / ml275,00
 
Ultra Rainbow Fluorecent Calibration Particles
New flow cytometers with increasing numbers of fluorescent channels in the UV and Far Red and the availablity of corresponding fluorescent conjugates are now available.
In order to keep up, we have developed the Ultra Rainbow Fluorescent Particles with enhanced UV and Far Red fluorecence intensity. The beads contain a single peak and are designed for checking the optical alignment of any flow cytometer in all channels.
Cat. #Rainbow Flour. Calibration particlesSizeµ/ unitBeadsEuroAnzahl
P-URFP-1.01 peak1,0-1,4/ 2 ml10E7 / ml239,00
P-URFP-3.01 peak3,0-3,4/ 2 ml10E7 / ml239,00
P-URFP-3.0-201 peak3,0-3,4/ 20 ml10E7 / ml1.935,00
P-URFP-3.81 peak3,5-3,9/ 2 ml10E7 / ml239,00
P-URFP-3.8Amid range intensity3,5-3,9/ 5 ml10E7 / ml545,00
P-URFP-8.01 peak8,0-12,0/ 2 ml10E7 / ml279,00
P-URFP-13.01 peak13,0-17,9/ 2 ml10E7 / ml279,00
P-URFP-18.01 peak18,0-24,9/ 2 ml10E7 / ml285,00
P-URFP-25.01 peak25,0-35,0/ 2 ml10E7 / ml285,00
 
COMPtrol Particles
The COMPtrol particles are coated with Goat anti-Mouse Ig (H&L). They bind to fluorochrome-conjugated monoclonal mouse antibodies used during cell staining.
These particles provide a method for the quality control of these conjugates. They are also used ti aid in setting proper compensation to reduce the cross-talk between the channels of multicolor flow cytometers.
Cat. #COMPtrol KitsSizeµ/ unitEuroAnzahl
P-CMIgP-30-2KGaM Ig (H&L) 1x10E7 / ml3,0-3,4/ 2 x 5 ml240,00
P-CMIgP-50-3KGaM Ig (H&L) 1x10E7 / ml5,0-5,5/ 3 x 5 ml240,00
 
Drop Delay Calibration Particles
The Drop Control Calibration particles are fluorescent particles to aid in the determination of the drop delay value for flow cytometer sorters with the appropriate attachement. As a result of using the Drop Delay Calibration particles and the appropriate attachment, the accuracy and productivity during sorting is enhanced
Cat. #DescriptionSizeµ/ unitEuroAnzahl
P-DDCP7.0Drop Delay Calibration, 10E8/ml6,0-8,0/ 2 ml220,00
 
Rainbow Calibration Particles
Our calibration particles are designed for routine calibration of flow cytometers and are used extensivly for QC and long term performance tracking of flow cytometers. They can also be used for routine alignment and calibration in fluorescence and confocal fluorescence microscopy. All calibration particles are supplied in dropper bottles containing 5 x10 6 particles.
Rainbow Calibration Particles are a mixture of fluorophores that allows exitation at any wavelength from 365 to 650nm. It enables calibration of all channels of the flow cytometer with same set of particles in similiar size, but with different intensities.
Rainbow Fluorescent Particles are similiar to the above described, exept these represent single peak, uniform size particles. The Rainbow fluorescent Particles are usually the brightest peak of the corresponding Calibration Particles with the exeption of P-RFPM-3.0, which has fluorescent intensity of the stained cells. Since these particles contain single peak with very small flluorescence and size CV, they are very useful in the alignment of the optical system of the flow cytometer.
Yellow Calibration Particles are designed for routine calibration of the fluorescin channel. They contain a set of similiar size Yellow Particles with different fluorescence intensities.
Rainbow Linear Calibration Particles are designed to check the linearity of the PMT in the linear scale of all available channels.They are also useful for quantifying the DNA content of cells stained with Hoechst dye, DAPI, Ethidium bromide, Propidium bromide, YOYO or TOTO.
Allophycocyanin Calibration Particles contain a mixture of 3.0µ fluorescent particles with different intensity in Allophycocyanin channel and can be exited with either a He-Ne laser at 632 nm or a diode laser at 635nm.
Blank Calibration Particles are the blank peak of the corresponding Rainbow Calibration Particles. These particles can be used to set the threshold of the instrument in each channel.
Cat. #Rainbow Calibration particlesSizeµ/ unitEuroAnzahl
P-RCP-2.04 peaks1,8-2,2/ 5ml255,00
P-RCP-3.06 peaks3,0-3,4/ 5ml255,00
P-RCP8-3.08 peaks3,0-3,4/ 5ml210,00
P-RCP-3.54 peaks3,5-4,0/ 5ml299,85
P-RCP-6.06 peaks6,0-6,5/ 5ml265,00
Rainbow Fluorescent particles
P-RFP-2.0Brightest peak in P-RCP-2.01,8-2,2/ 5ml265,85
P-RFP-3.0Brightest peak in P-RCP-3.03,0-3,4/ 5ml288,85
P-RFPM-3.0Mid range FL1 fluorescence3,0-3,4/ 5ml288,85
P-RFP-3.5Brightest peak in P-RCP-3.53,5-4,0/ 5ml299,85
P-RFP-6.0Brightest peak in P-RCP-6.06,0-6,5/ 5ml299,85
P-RFP-7.0Brightest peak in P-RCP-7.06,5-8,0/ 5ml299,85
P-YCP-7.0Yellow Calibration particles, 5 peaks6,5-8.0/ 5ml315,60
P-RLCP-3.0Rainbow Linear Calibration particles, 5 peaks3,0-3,4/ 5ml299,20
P-ACP-3.0Allophycocyanin Calibration particles3,0-3,4/ 2x5ml299,85
Blank Calibration particles
P-BCP-2.0Blank Peak in P-RCP-2.01,8-2,2/ 5ml152,85
P-BCP-3.0Blank Peak in P-RCP-3.03,0-3,4/ 5ml152,85
P-BCP-3.5Blank Peak in P-RCP-3.53,5-4,0/ 5ml152,85
P-BCP-6.0Blank Peak in P-RCP-6.06,0-6,5/ 5ml152,85
P-BCP-7.0Blank Peak in P-RCP-7.06,5-8,0/ 5ml152,85
Available Data Sheets regarding Flow Cytometry on Request.
1. Calibration and performance tracking of Flow Cytometer using Calibration Particles
2. Measuring Molecules of Equivalent Fluorescin ( MEFL ), PE ( MEPE ) and RPE-CY5 (MEPCY ) using Rainbow Calibration Particles.
 
Fluorescent Particle Kits
The Pink Fluorescent Particle Kits consist of either 3.6µ or 2,8µ Fluorescent Particles with twelve different fluorescence intensities in PE channel. They are designed to simplify the multiplex assay development using any flow cytometer. Low cost FITC conjugates can be used for detection. They are supplied as kits of either six odd numbered peaks or six even numbered peaks. Four different Pink Fluorescent Particle Kits are available for different applications.
The Yellow Fluorescent Particle Kits consist of 2,8µ and 3,6µ Yellow Fluorescent Particles in multiple fluorescence intensities in FITC channels. These two kits can be used to develop multiplex assays using either PE or PE-Cy5 tracers for detection.
Cat. #Description% w/v / pack sizeEuroAnzahl
P-PO3.6KitPink-odd 6 peaks, 3,6µ0,5% / 6 x 1ml572,60
P-PE3.6KitPink even 6 peaks, 3,6µ0,5% / 6 x 1ml572,60
P-GAMPO3.6KitGoat-anti-Mouse Pink-odd 6 peaks, 3,6µ0,1% / 6 x 1ml572,60
P-GAMPE3.6KitGoat-anti-Mouse Pink-even 6 peaks, 3,6µ0,1% / 6 x 1ml572,60
P-CPO2.8KitCarboxyl Pink-odd 6 peaks, 2,8µ0,25% / 6 x 1ml572,60
P-CPE2.8KitCarboxyl Pink-even 6 peaks, 2,8µ0,25% / 6 x 1ml572,60
P-S-AO2.8KitS-Avidin Pink-odd 6 peaks, 2,8µ0,1% / 6 x 1ml572,60
P-S-AE2.8KitS-Avidin Pink-odd 6 peaks, 2,8µ0,1% / 6 x 1 ml572,60
P-YO2.8KitYellow-odd 6 peaks, 2,8µ0,5% / 6 x 1 ml572,60
P-YE2.8KitYellow-even 6 peaks, 2,8µ0,5% / 6 x 1ml572,60
P-Y9-2.8KitYellow 9 peaks, 3,6µ0,5% / 6 x 1ml713,20
 
Fluorescent Polystyrene Particles
Cat. #Type & ColorSize in µ% w/vpacksizeEuroAnzahl
PFP-00552Yellow0,04 –0,0612ml139,00
PFP-0252Yellow0,1 – 0,312ml139,00
PFP-0245Light yellow0,1 – 0,312ml139,00
PFP-0552Yellow0,4 – 0,612ml139,00
PFP-0545Light yellow0,4 – 0,612ml139,00
PFP-0852Yellow0,7 – 0,912ml139,00
PFP-0845Light yellow0,7 – 0,912ml139,00
PFL-2045Light yellow, low intensity1,7 – 2,212ml159,00
PFP-2045Light yellow, medium intensity1,7 – 2,212ml159,00
PFH-2045Light yellow, high intensity1,7 – 2,212ml159,00
PFL-2052Yellow, low intensity1,7 – 2,212ml159,00
PFH-2052Yellow, high intensity1,7 – 2,212ml159,00
PFP-3052Yellow2,5 – 3,412ml179,00
PFP-4052Yellow3,5 – 4,512ml179,00
PFP-6052Yellow6,0 – 7,912ml192,00
PFP-10052Yellow10,0 – 14,012ml235,00
PFP-15052Yellow15,0 – 19,012ml259,00
PCFP-00546Light yellow carboxylated0,04 – 0,0812ml179,00
PCFP-00552Yellow carboxylated0,04 – 0,0812ml179,00
PCFP-0252Yellow carboxylated0,09 – 0,312ml179,00
PCFP-0552Yellow carboxylated0,4 – 0,612ml179,00
PCFP-0852Yellow carboxylated0,7 – 0,912ml179,00
PCFL-0852Yellow carboxylated, low intensity0,7 – 0,912ml179,00
PCFP-2052Yellow carboxylated1,7 – 2,212ml192,00
PCFH-5052Yellow carboxylated, high intensity5,0 – 5,90,52ml192,00
PCFP-5052Yellow carbox., medium intensity5,0 – 5,90,52ml192,00
PCFL-5052Yellow carboxylated, low intensity5,0 – 5,90,52ml192,00
PAFP-0252Yellow amino0,09 – 0,312ml179,00
PAFP-0552Yellow amino0,4 – 0,612ml179,00
PAFP-0852Yellow amino0,7 – 0,912ml179,00
Cat. #Type & ColorSize in µ% w/vpacksizeEuroAnzahl
PFP-00556Nile red0,04 –0,0612ml139,00
PFP-0256Nile red0,1 – 0,312ml139,00
PFP-0856Nile red0,7 – 0,912ml139,00
PFL-2056Nile red, low intensity1,7 – 2,212ml159,00
PFH-2056Nile red, high intensity1,7 – 2,212ml159,00
PFP-2056Nile red1,7 – 2,212ml159,00
PFP-3056Nile red2,5 – 3,412ml179,00
PFP-4056Nile red3,5 – 4,512ml179,00
PFP-6056Nile red6,0 – 7,912ml192,00
PFL-6056Nile red, low intensity6,0 – 7,912ml192,00
PFP-9056Nile red8,0 - 9,912ml259,00
PFH-10056Nile red, high intensity10,0 – 14,012ml235,00
PFP-10056Nile red10,0 – 14,012ml235,00
PFP-15056Nile red15,0 – 19,012ml259,00
PCFP-00556Nile red carboxylated0,04 – 0,0812ml189,00
PCFP-0256Nile red carboxylated0,09 – 0,312ml189,00
PCFP-0556Nile red carboxylated0,4 – 0,612ml189,00
PCFP-0856Nile red carboxylated0,7 – 0,912ml189,00
PCFH-5056Nile red carbox., high intensity5,0 – 5,90,52ml199,00
PCFP-5056Nile red carbox., medium intensity5,0 – 5,90,52ml199,00
Cat. #Type & ColorSize in µ% w/vpacksizeEuroAnzahl
PFP-00558Pink0,04 –0,0612ml139,00
PFP-0558Pink0,4 – 0,612ml139,00
PFP-0858Pink0,7 – 0,912ml139,00
PFP-2058Pink1,7 – 2,212ml159,00
PFP-4058Pink2,5 – 4,512ml179,00
192,00Pink6,0 – 7,912ml192,00
PCFP-00558Pink carboxylated0,04 – 0,0812ml179,00
PCFP-0558Pink carboxylated0,4 – 0,612ml179,00
PCFP-0858Pink carboxylated0,7 – 0,912ml179,00
PCFP-2058Pink carboxylated1,7 – 2,212ml192,00
PCFH-5058Pink carbox., high intensity5,0 – 5,90,52ml192,00
PCFP-5058Pink carbox., medium intensity5,0 – 5,90,52ml192,00
PCFL-5058Pink carbox., low intensity5,0 – 5,90,52ml192,00
Cat. #Type & ColorSize in µ% w/vpacksizeEuroAnzahl
PFP-00562Purple0,04 –0,0612ml139,00
PFP-0262Purple0,1-0,312ml139,00
PFP-0562Purple0,4 – 0,612ml139,00
PFP-0862Purple0,7 – 0,912ml139,00
PFL-2062Purple, low intensity1,7 – 2,212ml159,00
PFP-2062Purple, medium intensity1,7 – 2,212ml159,00
PFH-2062Purple, high intensity1,7 – 2,212ml159,00
PFP-4062Purple2,5 – 4,512ml179,00
PCFP-0562Purple carboxylated0,4 – 0,612ml179,00
PCFP-0862Purple carboxylated0,7 – 0,912ml179,00
PCFP-2062Purple carboxylated1,7 – 2,212ml192,00
Cat. #Type & ColorSize in µ% w/vpacksizeEuroAnzahl
PFP-00570Sky blue0,04 –0,060,252ml139,00
PFP-0270Sky blue0,1-0,30,252ml139,00
PFP-0570Sky blue0,4 – 0,61,02ml139,00
PFP-0870Sky blue0,7 – 0,912ml139,00
PFP-2070Sky blue1,7 – 2,212ml159,00
PFH-2070Sky blue, high intensity1,7 – 2,20,22ml159,00
PFP-3070Sky blue2,5 – 3,40,22ml179,00
PFP-4070Sky blue3,5 – 4,50,22ml179,00
PFL-4070Sky blue, low intensity2,5 – 4,51,02ml179,00
PFP-15070Sky blue15,0-19,00,22ml259,00
PCFP-0570Sky blue carboxylated0,4 – 0,60,252ml179,00
PCFP-0870Sky blue carboxylated0,7 – 0,912ml179,00
These particles can also be offered as 10% suspension, when higher concentrations for production are necessarry. Also available carboxylated in 10% suspension, Please ask for quotation.
 
PMMA ( Pylymethylmethacrylate ) Particles, supplied in 10% suspension
These beads have a typical wider size distribution for bigger sized beads
Cat.#size/quantitychemistryEuroAnzahl
PPMMA-10.010,0µ/ 15mlplain340,00
PPMMA-20.020,0µ/ 15mlplain452,50
PPMMA-50.050,0µ/ 15mlplain452,50
PPMMa-100.0100,0µ/ 15mlplain452,50
 
Large Size Particles
In the following chapter we offer some beads of larger sizes but with relative narrow size distribution. The offered quantities are laboratory quantities and all beads can be offered as bulk quantities also. The beads are used for different applications, e. g. manufacturing of implatates( pmma-beads and pharmaceutical synthetic research (polystyrene beads).
Cat. #size materialquantityEuroAnzahl
PLPS-1513 -18µPolystyrene, crosslinked10 ml/2.5%219,00
PLPS-2724 - 30µPolystyrene, crosslinked10 ml/2.5%299,00
PLPS-4038 - 45µPolystyrene, crosslinked10 ml/5.0%169,00
PLPS-5552 - 63µPolystyrene, crosslinked10 ml/5.0%169,00
PLPS-10090 -105µPolystyrene, crosslinked10 ml/5.0%169,00
PLPS-135125 -149µPolystyrene, crosslinked10 ml/5.0%169,00
PLPS-230210 - 250µPolystyrene, crosslinked10 ml/5.0%169,00
PLPM-300200 - 400µPMMA10g29,00
PLPM-400400 - 500µPMMA10g29,00
PLPM-600500 - 600 µPMMA10g29,00
PLPM-800700 - 1000µPMMA10g29,00
 
Melamine Microparticles - powdered
The melamine microparticles ( polymethylenemelamine particles) are made from crosslinked melamine and have some advantages depending on the application compared to polystyrene particles. They have a higher density ( 1,51g/cm3), are very stable, can be stored unlimited, can be resuspended in water, they do not quench in most organic solvents and are heat resistant up to 200°C. The melamine microparticles are hydrophilic and monodisperse in water (CV 1-2%). The refractive index is 1,68.
Cat. #Size/ quantitychemistryEuroAnzahl
PMa-1.01,0µ/ 750mg 250,00
PMa-1.01,0µ/ 1,5g 475,00
PMa-2.02,0µ/ 750mg 250,00
PMa-2.02,0µ/ 1,5g 475,00
PMa-3.03,0µ/ 750mg 250,00
PMa-3.03,0µ/ 1,5g 475,00
PMa-4.04,0µ/ 750mg 250,00
PMa-4.04,0µ/ 1,5g 475,00
PMa-5.05,0µ/ 750mg 289,00
PMa-5.05,0µ/1,5g 553,00
PMa-6.06,0µ/ 750mg 289,00
PMa-6.06,0µ/ 1,5g 553,00
PMa-7.07,0µ/ 750mg 289,00
PMa-7.07,0µ/ 1,5g 553,00
PMa-8.08,0µ/ 750mg 289,00
PMa-8.08,0µ/ 1,5g 553,00
PMa-9.09,0µ/ 750mg 289,00
PMa-9.09,0µ/ 1,5g 553,00
PMa-10.010,0µ/ 750mg 310,00
PMa-10.010,0µ/ 1,5g 565,00
 
Fluorescent Melamine Microparticles
These fluorescent microparticles have same basic characteristics as the powdered melamine microparticles. They are useful e.g. in diagnostic assays, protein binding procedures, size standards in microscopy applications as tracers and indicators, wherever a single particel must be identified in mixt medium. All fluorescent microparticles are supplied as 2,5% aqueous suspension. We offer a wide range of sizes with differnt fluorescent dyes.
FITC particles: Excitation 490nm; Emmission 525nm
Rhodamine B: Excitation 540nmn; Emmission 625nm
Nil blue: Excitation 633nm,; Emmission 672
Additional available microspheres are: Acridine orange and Ethidium bromide marked microspheres, please ask for available size and price.
Cat. #Size/ quantitydyeEuroAnzahl
PMaF-1.01,0µ/ 5mlFITC355,00/5ml…539,00/15ml
PMaRhB-1.01,0µ/ 5mlRhodamine B355,00/5ml…539,00/15ml
PMaNB-1.01,0µ/ 5mlNile blue355,00/5ml…539,00/15ml
PMaF-2.02,0µ/ 5mlFITC355,00/5ml…539,00/15ml
PMaRhB-2.02,0µ/ 5mlRhodamine B355,00/5ml…539,00/15ml
PMaNB-2.02,0µ/ 5mlNile blue355,00/5ml…539,00/15ml
PMaF-3.03,0µ/ 5mlFITC355,00/5ml…539,00/15ml
PMaRhB-3.03,0µ/ 5mlRhodamine B355,00/5ml…539,00/15ml
PMaNB-3.03,0µ/ 5mlNile blue355,00/5ml…539,00/15ml
PMaF-4.04,0µ/ 5mlFITC355,00/5ml…539,00/15ml
PMaRhB-4.04,0µ/ 5mlRhodamine B355,00/5ml…539,00/15ml
PMaNB-4.04,0µ/ 5mlNile blue355,00/5ml…539,00/15ml
PMaF-5.05,0µ/ 5mlFITC383,75/5ml 588,00/15ml
PMaRhB-5.05,0µ/ 5mlRhodamine B383,75/5ml 588,00/15ml
PMaNB-5.05,0µ/ 5mlNile blue383,75/5ml 588,00/15ml
PMaF-6.06,0µ/ 5mlFITC383,75/5ml 588,00/15ml
PMaRhb-6.06,0µ/ 5mlRhodamine B383,75/5ml 588,00/15ml
PMaNB-6.06,0µ/ 5mlNile blue383,75/5ml 588,00/15ml
PMaF-8.08,0µ/ 5mlFITC383,75/5ml 588,00/15ml
PMaRhB-8.08,0µ/ 5mlRhodamine B383,75/5ml 588,00/15ml
PMaNB-8.08,0µ/ 5mlNile blue383,75/5ml 588,00/15ml
PMaF-10.010,0µ/ 5mlFITC415,00/5ml 620,00/15ml
PMaRhB-10.010,0µ/ 5mlRhodamine B415,00/5ml 620,00/15ml
PMaNB-10.010,0µ/ 5mlNile blue415,00/5ml 620,00/15ml
 
NIST Certified Particles
Our Particle size standards are made from Polystyrene (Ps) or crosslinked Melamin (Mf) . We offer this superior grade of particles in the widest range from 0,1µ to 15µ of certified particles available today. Each lot of the NIST Certified Particles is characterized by documented methods, based on the NIST (National Institute of Standards and Technology) and. CRB ( Community Bureau of Reference ) as TEM, REM and Coulter Counter.

For each particle size standard we supply a certificate of analysis, which documents the most important characteristics and physical data like number of particles, particle size, standard deviation ( SD ), Coefficient of Variance ( CV ), specific weight, refractive index, bead density and more.

The particle size standards are supplied as 2% or 3% suspension in deionized water. The CV is depending on each size at 1 - 3,5%
Further intermediate sizes are available on requets.
Cat. #.Size /quantity/concentrationmaterialEuroAnzahl
PNPs-0.10,1µ/10ml/2%Ps210,00
PNPs-0.50,5µ/10ml/2%Ps210,00
PNPs-1.01,0µ/10ml/2%Ps210,00
PNPs-1.51,5µ/10ml/2%Ps259,00
PNMf-2.02,0µ/10ml/2%Mf259,00
PNMf-2.52,5µ/10ml/2%Mf259,00
PNMf-3.03,0µ/10ml/2%Mf259,00
PNMf-3.53,5µ/10ml/2%Mf259,00
PNMf-4.04,0µ/10ml/2%Mf259,00
PNPs-4.04,0µ/10ml/2%Ps259,00
PNMf-4.54,5µ/10ml/2%Mf259,00
PNMf-5.05,0µ/10ml/2%Mf259,00
PNMf-5.55,5µ/10ml/2%Mf259,00
PNMf-6.06,0µ/10ml/2%Mf259,00
PNMf-7.07,0µ/10ml/2%Mf259,00
PNMf-8.08,0µ/10ml/2%Mf259,00
PNMf-9.09,0µ/10ml/2%Mf259,00
PNMf-10.010,0µ/10ml/2%Mf295,00
PNPs-10.010,0µ/20ml/3%Ps365,00
PNPs-15.015,0µ/20ml/3%Ps365,00
PNPs-20.020,0µ/20ml/3%Ps365,00
PNPs-40.040,0µ/20ml/3%Ps385,00
PNPs-50.050,0µ/20ml/3%Ps385,00
PNPs-75.075,0µ/20ml/3%Ps395,00
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