Kisker Biotech
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DNA-Workstation & Hybridisation

 
DNA-Workstation I
The DNA-Workstation is an UV sterilisation cabinett to decontaminate reagents and equipment prior to carrying out PCR reactions. The powerful 15 watt UV light (254 nm) of the DNA-Workstation denatures nucleic acids in 5-30 minutes making them unsuitable for amplification and therefore eliminating any possible cross contamination. The DNA-Workstations are equipped with a white lamp for illumination. The 10 mm acrylic walls or 8 - 10 mm glass walls also acts as an efficient shield of beta-radioactive emissions. The DNA-Workstations stand on an easy to clean stainless steel tray.

Available models:

L020C: acryl side walls, 1 x 15 watt UV-lamp (254 nm), 1 x 15 watt white light lamp and 1 x UV/Air cleaner (254 nm), which circulates the air along the UV-lamp for continues denaturation, lower part front to turn up.
Dimensions: 69 x 51 x 57 cm (WxDxH)
Weight: 25 kg

L020-GC: glass sides, which are resistant to UV-cracking and can be cleaned with the medium of choice, equipped with 1 x 15 watt UV-lamp (254 nm), 1x 15 watt white light lamp and 1 x UV/Air cleaner (254 nm).
Dimensions: 69 x 51 x 57 cm (WxDxH)
Weight: 35 kg
Cat. #DescriptionPowerEuroUnits
L020-CDNA-Workstation, Acryl with UV/Air-Cleaner220Volt/50Hz1.595,00
L020-GCDNA-Workstation, Glass with UV/Air Cleaner220Volt/50Hz1.865,00
 
DNA-Workstation I-XL
This XL-version of the workstation shows the same advantages like DNA-workstation I.
In comparison with the standard version the XL-version provides a working area of 120 x 52 cm and 3 electrical socket for the use of further laboratory equipment inside the workstation.

L020-XL-GC:
The base of the front-screen can be locked in 3 positions
2 x 30 watt UV-lamp (254 nm)
1 x 30 watt whitelight lamp
1 x 30 watt UV/Air-Cleaner (254 nm)
Dimensions: 124,5 x 58 x 58,5 cm (BxTxH)
Weight: 58 kg
Cat. #DescriptionPowerEuroUnits
L020-XL-GCDNA-Workstation, Glass with UV/Air Cleaner220Volt/50Hz2.590,00
 
UV-Cleaner, the new combination of Laminar Flow and UV-light for Clean Rooms
This UV-Cleaner provides DNA-fragment-free laboratories without direct UV-light. The air is constantly flowing through the instrument, which is equipped with a fan, an UV-lamp and a dust filter. Inside the box the density of the UV-light is increased 1000 fold, sufficient to inactivate DNA complete. Also microbiological contaminations are reduced strongly.
The UV-Laminar-Flow is able to clean 25 m3 of air/ hour to provide DNA free laboratory of 25 m3 within 3 hours.

Further advantages:
no HEPA-filters to change
ozon-free high density UV decontamination
noise free, low energy consumption
long living UV-lamps (8.000 hr)
compact for wall fixation


The instrument is recommended for clean room laboratories as PCR, DNA-Analysis, Microbiology, Virology, Tissue Culture or other rooms, where a contamination free area is needed. After 12 hours of air cleaning, the UV-Laminar Flow provides an estimated reduction of the colony forming unit to 80 - 100 CFU/m3 air
the cleaner can be mounted on the wal or a stand is available optional

L046-M
weight: 4 kg, dimensions 82 x 92 x 600 mm
power 220/240 volt, 50/60Hz, 30 watt.
Cat. #DescriptionCapacity/hrEuroUnits
L046-MUV-Cleaner25m3399,00
L046-STStand for Cleaner 95,00
 
DNA-Workstation II
This DNA-Workstation avoids cross-contamination by suction of the air and ducting it through an activated charcoal filter. All particles are captured and will not reach normal lab air.
The DNA-Workstation is equipped with an UV-lamp (254 nm) and filtercassette. The hour-counter shows the overall working time. The activated charcoal filter should be replaced every 1000 working hours. The work surface is made from stainless steal and easy to clean. The airflow is adjustable from 0 - 100%. The DNA-Workstation is available in 3 versions. The perspex cover can be moved 100mm backwards (exept USA version).

Specification: electr. power: 220volt/50Hz/80watt
Dimensions of working space: 470 x 300mm (W x D)
suction space: 410 x 240mm (W x D)
Exterior dimensions Standard-version: 485 x 540 x 250mm (W x D x H)
Sydney-version: 485 x 540 x 465mm (W x D x H)
USA-version : 485 x 540 x 600mm (W x D x H)
Cat. #DescriptionEuroUnits
L016DNA-Workstation (Standard)1.598,00
L016-SYDNA-Workstation (Sydney)1.598,00
L016-USDNA-Workstation (USA)1.955,00
L016-AFact.charcoal filter40,90
L016- UVUV-lamp45,00
 
BIO-LINK-Crosslinker
BIO-LINK 254 nm provides a fast and reliable method, to fix DNA or RNA onto nylon- or nitrocellulose membranes. The crosslinking requires only a few seconds for a stable fixation between DNA/RNA and the membran. This method avoids a loss of samples through later hybridisation. The signal intensity is manifold increased (5 - 10 times) compared to the traditional and time consuming baking method (two hours at 80°C ). The BIO-LINK is equipped with five tubes of 8 Watt and also available in 312 nm and 365 nm. The big volume chamber (14,5 x 33,0 x 26 cm / HxDxW) offers space for bigger tubes and plates.
UV-energy supply can be set precise. BIO-LINK is equipped with an UV-energy programming system with a time integrator, which constantly monitors the UV-light emission.

Further technical details:
  • 9 presetted energy-/time programs or manual configuration

  • max. UV-exposition in two ranges:0,025 - 9,999 joules and 0-99,99 joules

  • high reproducability by UV-sensors

  • large, user friendly keybord with LED-display
  • internal safety interlock

  • timer up to 999,9 minutes

  • Dimensions: 30,5 x 36 x 35 cm (HxDxW), weight: 10,5 kg


  • In addition to the crosslinking of nucleic acids BIO-LINK can be used for:
  • "Nicking" of DNA in agarose-geles

  • partial restriction endonuclease digestion by formation of thymine dimers

  • Rec-A mutation-screening

  • elimination of PCR-contamination

  • Cat. #DescriptionEuroUnits
    BLX-254BIO-LINK 254 nm1.085,00
    BLX-312BIO-LINK 312 nm1.085,00
    BLX-365BIO-LINK 365 nm1.085,00
     
    Nitrocellulose-Membrane
    Membrane of 100% pure nitrocellulose for transfer of proteins and nucleic acids. It shows high binding capacity (80 - 100 µg / cm2) and a low background. Pore size 0,45 µm is used for standard applications. The 0,2 µm pore size is recommended for transfer of low molecular weight proteins (< 20 kDa) and nucleic acids to avoid loss of sample during transfer.
    Cat. #DescriptionSizePack sizeEuroUnits
    MNC-0.2Nitrocellulose-Membran 0,2µ33 x 300 cm 1 Roll169,00
    MNC-0.4Nitrocellulose-Membran 0,45µ33 x 300 cm 1 Roll169,00
    MBP-0.17Blotting-Paper 0,17 mm46 x 57 cm 100 sheets109,00
    MBP-0.34Blotting-Paper 0,34 mm46 x 57 cm 100 sheets169,00
     
    Incubator and Hybridisation ofen
    The incubator is a compact bench-top unit, which is available in different configurationes. It can be supplied as incubationofen with and without rotissery or rockingplatform. A large usable space and a big LED Display garant easy setting and reading of the microprocessor controled temperature and speed. A key switch is used to select rotissery or shaking mode at the combination version L018-HY-RK.

    Specificationes:
    temperature range RT - 80°C, temerature accuracy 0,2°C
    speed rotissery 5 - 100 rpm
    speed rocking platform 5-100 rpm
    speed orbital shakingplatform 5-200 rpm
    speed Vortexer 5-1.500 rpm
    dimensions of platformen 200 x 270 mm
    inner dimensions 340 x 260 x 250 mm, overall dimensions 360 x 440 x 430 mm, weight 25,0 kg
    Cat. #DescriptionEuroUnits
    L018Incubator980,00
    L018-RKIncubator with rocker-platform1.395,00
    L018-OSIncubator with Orbital-platform1.595,00
    L018-VSIncubator with Vortex für micro/deep well plates1.595,00
    L018-HYHybridisation ofen with 1 rotissery selected from below 1.395,00
    L018-HY-RKHybridisation ofen with rotissery and rockingplatform1.645,00
    L018-8Rrotissery for 8 tubes of 35-40 mm diameter235,00
    L018-16Rrotissery for 16 x 50 ml tubes, conical235,00
    L018-24Rrotissery for 24 x 15 ml tubes, conical235,00
     
    Digi-Image, Geldocumentation-System
    Digi-Image Geldocumentation-System is a compact "Low-Budget"-System, which works without the need of a space consuming PC in the laboratory. The digital 8 megapixel camera offers excellent resolution. Pictures are viewed direct through a 8" TFT colour monitor. If necessary, pictures can be stored on a memory card (1 GB included with multicard-reader) to transfer data to a PC for further analysis. If purchased with the corresponding UV-Transilluminator (cat.# DG-UV-20M) the safety switch turn off the transilluminator when the door is opened. Also the internal light is activated for convenient gel manipulation. A thermoprinter is also available.

    Digi-Imagesystem:
    Specifications dark hood
    Dimensions: 29 x 22 x 32 cm (B x T x H)
    8"TFT-colour monitor (resolution: 640 x 480 pixel)
    Multi power source for camera, whitelight and monitor
    video- u.audio-input

    Specifications camera:
    8-megapixel
    resolution: 640 x 480 to 3.264 x 2.448 pixel
    8-fold optical zoom 8,9 - 71,2 mm
    Autofocus: TTL (Contract-detect through-the-lens)

    Digi-Imagesystem also available with UV-transilluminator (DG-S_UV)

    Specifications UV-transilluminator (312 nm):
    filter: 21x 21 cm (EtBr), High/Low-intensity-switch
    UV-protection shield: 330 x 250 mm
    overall dimensions: 340 x 295 x 100 mm, weight: 7,5 kg
    Cat. #DescriptionEuroUnits
    DG-SImage-System inclusive 8-megapixel digital camera, 8\" TFT colour monitor, camera hood and EtBr-Filter 2.290,00
    DG-S-UVImage-System inclusive 8-megapixel digital camera, 8\" TFT colour monitor, camera hood and UV-transilluminator (312 nm) 2.890,00
    DG-UV-20MUV-transilluminator (312 nm), 20 x 20 cm (EtBr), dual intensity with UV-protection shield750,00
    DG-S/TPs/w Thermoprinter Mitsubishi, P93-E1.115,00
    DG-EBFFilter (55 mm) -Ethidiumbromide for the camera235,00
    DG-SYFFilter - SYBR® -Green for the camera235,00
    DG-K61BThermopaper Quality Standard, width: 110 mm, length: 21 m, VE = 4 rolls49,90
    DG-K65HMThermopaper Quality \"High Density\" width: 110 mm, length: 21 m, VE = 4 rolls99,90
     
    Flexi, Geldocumentation-System
    This Flexi 5000 geldocumentation-System is based on a digital-camera with is fully PC-controlled by the software Argus X1. It“s a extremly user friendly system with all advantages of a digital-camera - for example the high image resolution. For the administration of the data amounts it contains a integrated data base.

    Basic package Flexi 5000: Digital-camera, close-up lense, filter (EtBr or SyBr Green), controlling cable, controlling software Argus X1

    Dark hood UDH-10
    UV-transilluminator
    either UTR-20M (312 nm) or UTR-20L (365 nm).

    Specifications camera:
    Chip: CCD-Chip
    Resolution: 5 mio. pixel
    Greyscale: 8 bit (256)
    Colours: 24 Bit (16,7E6)
    Zoomobjektive: motor zoom
    max. integration time: 10 sec.

    Specifications dark hood:
    Set-top dark hood UDH-10
    stand area (W x L) 30 cm x 25 cm

    Specifications UV-transilluminator:
    optionally UTR-20M (312 nm) or UTR-20L (365 nm)
    filter-size: 20 x 20 cm
    6 tubes with each 8 Watt

    Basic package Flexi 5000-S:
    Digital-camera, close-up-lense, filter (EtBr or SyBr Green), controlling cable, controlling software Argus X1

    Optionally there will be offered a thermoprinter for both systems.
    Cat. #DescriptionEuroUnits
    Flexi 5000Image-System Flexi incl. digital-camera with controlling software Argus X1, set-top dark hood UDH-10 and UV-transilluminator UTR-20M or UTR-20L2.924,00
    Flexi 5000-SImage-System Flexi incl. Digital-camera with controlling software Argus X11.995,00
    Flexi 5000-TPs/w Thermoprinter Mitsubishi, P93-DW1.255,00
     
    UV - Transilluminators
    homogenous lightning by 6, 8 or 12 Tubes (depending on model), long tube shelflife through integrated cooling fun, electronical start, models with stepless choosable intensity of the tubes (10-100%), UV-protection shield (optional) usable angle 0-90°, high quality construction in compact design,

    The listed illuminators are useful for following dyes:
    254 nmHoechst Blue, Radiant Red, Chemiefluoreszenz
    312 nmEthidiumbromid, SYBR Orange, Fluorescein,Texas Red, SYBR Red
    365 nmSYBR Green, GFP, CHO, Hoechst 33258,Fluorescamine, EBFP
    BluelightGel Star, SYBR Green, SBBR Gold, AttoPhos,PicoGreen, NBD, Acridine Orange, EGFP
    WhitelightCoomassie Blue, Silver Stain, Film Imaging
    Accessories: UV-protection shield, replacement tubes and glases, UV-protection glas and face shield
     
    Transilluminators
    Cat. #WavelengthNumber tubes x WattFilter sizeIntensity controlEuroUnits
    UTR-20S2548 x 820x2050 or 100%889,00
    UTR-20M3126 x 820x20none634,00
    UTR-20L3656 x 820x20none634,00
    UTR-30S25412 x 823x3050 or 100%1.039,00
    UTR-30M3128 x 823x30none824,00
    UTR-30L3658 x 823x30none824,00
    UTR-40S2546 x 1520x4050 or 100%1.429,00
    UTR-40M3126 x 1520x4050 or 100%1.329,00
    UTR-40L3656 x 1520x4050 or 100%1.329,00
    UTR-20S-V2548 x 820x2010-100%1.049,00
    UTR-20M-V3128 x 820x2010-100%989,00
    UTR-20L-V3658 x 820x2010-100%989,00
    UTR-30S-V25412 x 823x3010-100%1.249,00
    UTR-30M-V31212 x 823x3010-100%1.189,00
    UTR-30L-V36512 x 823x3010-100%1.189,00
    UTR-40S-V2546 x 1520x4010-100%1.589,00
    UTR-40M-V3126 x 1520x4010-100%1.499,00
    UTR-40L-V3656 x 1520x4010-100%1.499,00
    UTR-20S/M254/3124 x 8 / 4 x 820x2050 or 100%989,00
    UTR-20S/L254/3654 x 8 / 4 x 820x2050 or 100%989,00
    UTR-20M/L312/3654 x 8 / 4 x 820x2050 or 100%929,00
    UTR-30S/M254/3126 x 8 / 6 x 823x3050 or 100%1.259,00
    UTR-30S/L254/3656 x 8 / 6 x 823x3050 or 100%1.259,00
    UTR-30M/L312/3656 x 8 / 6 x 823x3050 or 100%1.169,00
    UTR-40S/M254/3123x15 / 3 x1520x4050 or 100%1.599,00
    UTR-40S/L254/3653x15 / 3 x1520x4050 or 100%1.599,00
    UTR-40M/L312/3653x15 / 3 x1520x4050 or 100%1.499,00
    UTR-20S/WL254/whitelight6 x 8 UV / 2 x 8 WL20x20none1.130,00
    UTR-20M/WL312/whitelight6 x 8 UV / 2 x 8 WL20x20none1.130,00
    UTR-20L/WL365/whitelight6 x 8 UV / 2 x 8 WL20x20none810,00
    WL-20whitelight8 x 820x20none489,00
    WL-30whitelight12 x 823x30none629,00
    BLT-20Bluelight, LED-Matrix 225 20x2050 or 100%795,00
    BLT-30Bluelight, LED-Matrix 3508 23x3050 or 100%979,00
     
    Accessories
    Cat. #DescriptionEuroUnits
    UVT-UVSUV-protectionshield47,00
    UVB-mBUV-glasses with ear piece35,00
    UVB-mGUV-glasses with rubber band35,00
    UV-VGUV-face shield74,90
     
    UV-Lamps
    We offer with this productline UV-lamps in different wavelengths. The well designed lamps are easy to handle and reliable.
    A special cost effective product are the lamps in combination of two different wavelengths.

    UV-lamps are recommended to use according to the wavelengths in following application areas

    254 nm: Elimination of PCR-contaminations, fluorochemistry, pesticide analysis, mutation studies, mykology, toxikology, mineralogy, polymer curing and control in industry
    The high intensity lamp UVL-100 is supplied with a separate power supply and is especially used for mutation studies and polymer curing.

    312 nm: DNA and RNA-electrophoresis, recognition of lanes in the gel.

    365 nm: cultivation of fungi, identification of bacteria, fluorochemistry, pesticide analysis, quality controle, food contamination, archeology and mineralogy.
    Cat. #WavelengthNumber of Tubes x WattDimensionen in mm WxDxHµW/CMEuroUnits
    UVL-4S2541 x 4199 x 85 x 60 340169,00
    UVL-4L3651 x 4199 x 85 x 60 400129,00
    UVL-4LSUVL-4LS2 x 4199 x 85 x 60 350/265350/265
    UVL-6S2541 x 6276 x 85 x 60 710247,00
    UVL-6M3121 x 6276 x 85 x 60 680247,00
    UVL-6L3651 x 6276 x 85 x 60 700135,00
    UVL-6LS365/2542 x 6276 x 85 x 60 610/400275,00
    UVL-6LM365/3122 x 6276 x 85 x 60 610/580275,00
    UVL-6MS312/2542 x 6276 x 85 x 60 580/400275,00
    UVL-8S2541 x 8351 x 85 x 60820304,00
    UVL-8M3121 x 8351 x 85 x 60790304,00
    UVL-8L3651 x 8351 x 85 x 60800209,00
    UVL-8LS365/2542 x 8351 x 85 x 60720/520329,00
    UVL-8LM365/3122 x 8351 x 85 x 60720/660329,00
    UVL-8MS312/2542 x 8351 x 85 x 60660/520329,00
    UVL-115S2541 x 15505 x 140 x 117 1.000417,00
    UVL-115M3121 x 15505 x 140 x 117 1.000417,00
    UVL-115L3651 x 15505 x 140 x 117 1.100247,00
    UVL-215S2542 x 15505 x 140 x 117 1.780675,00
    UVL-215M3122 x 15505 x 140 x 117 3.000675,00
    UVL-215L3652 x 15505 x 140 x 117 2.300429,00
    UVL-215LS365/2542 x 15505 x 140 x 117 1.350/1.100675,00
    UVL-215LM365/3122 x 15505 x 140 x 117 1.350/1.800675,00
    UVL-215MS312/2542 x 15505 x 140 x 117 1.800/930675,00
    UVL-1002541 x 100404 x 98 x 1973.4001.314,00
     
    Accessoires for UV-Lamps
    Cat. #DescriptionEuroUnits
    USVL-30Stand for UVL-115 and UVL-21582,00
    USVL-6Stand for 4 / 6 / 8 watt UV-lamps62,00
    USMAHHandle for 4 / 6 / 8 watt UV-lamps37,00
    USMUBracket for 4 / 6 / 8 watt UV-lamps23,00
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