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General Functions
Single Wavelength
The feature Single Wavelength allows simple absorbance (Abs) or transmission (%T) measurements at a single user defined wavelength. The result windows shows the amount of light passed through a sample relative to a reference.
Concentration
The feature Concentration determines the concentration of samples from a single wavelength reading as a Colorimetric assay.
The amount of light that has passed through a sample relative to a reference is multiplied by a factor to obtain the concentration. The factor may be a known figure, or may be calculated by the instrument by measuring a standard of known concentration.
The units to present the results are selectable (µg/ml, µg/µl, pmol/µl, mg/dl, mmol/l, µmol/l, g/l, mg/l, µg/l, U/l, %, ppm, ppb, conc or none).
Wavescan
The feature Wavescan records an absorption (Abs) or transmission (%T) spectrum between two user defined wavelengths, maximum range 190 nm to 1100nm.
The spectral plot allows simple identification of peak height and position.
Benefit
The curve interpretation of the entire spectrum provides most detailed information for a sample.
Kinetics
The feature Kinetics performs a colorimetric assay which follows the change in absorbance as a function of time at fixed wavelength.
Kinetic studies are routinely used for the enzymatic determination of compounds in food, beverage and clinical laboratories by measuring NAD/NADH conversion at 340nm.
The change of absorbance per minute (A/min), concentration (A/min x factor) and correlation coefficient (calculated from a best fit of the data points) are displayed.
Standard Curve
The feature Standard Curve is a colorimetric assay at a single wavelength based on a user programmed curve.
The preparation of a multi-point calibration curve from standards of known concentration to quantify unknown samples is a fundamental use of a spectrophotometer; this instrument has the advantage of being able to store this curve as a method, using up to 9 standards.
Besides, the data value used for the Standard Curve Calibration can be entered manually.
The units to present the results are selectable: µg/ml, µg/µl, pmol/µl, mg/dl, mmol/l, µmol/l, g/l, mg/l, µg/l, U/l, %, ppm, ppb, conc or none.
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