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Biotium : What fluorescent reagents for life science analysiss?

Founded in 2001 in California, Biotium develops innovative fluorescent dyes and reagents for molecular and cell biology. Its flagship solutions — GelRed/GelGreen for DNA, CF Dyes for labelling, EvaGreen for qPCR, NucView for apoptosis, PMAxx for vPCR — meet the demands of R&D, quality control, pharma, biotech and academic laboratories. Renowned for chemical safety, sensitivity and reproducibility, Biotium is a partner of choice for microscopy, cytometry and nucleic acid analysis.

Pioneers in fluorescence since 2001

Founded in 2001 in Fremont, California, Biotium was created by scientists with a clear mission: to make fluorescence higher-performing and safer for users and the environment. The launch of GelRed and GelGreen, non-mutagenic alternatives to ethidium bromide, marked a major turning point in DNA visualisation. The company then expanded its portfolio with CF Dyes (a palette of bright, photostable fluorophores), EvaGreen for qPCR/HRM, NucView for real-time caspase-3 monitoring, and PMAxx for viability PCR. Over the years, Biotium has strengthened its global presence through sustained R&D, tightly controlled manufacturing, and a rigorous quality system (including ISO 9001:2015 certification). Its commitment to safer chemistry – less hazardous waste, alternatives to UV and to toxic dyes – is now part of its identity.

Expertise and areas of specialisation

• Nucleic acids and qPCR: Biotium has redefined DNA detection with GelRed/GelGreen, sensitive and non-mutagenic, blue-light compatible to preserve samples. The AccuGreen/AccuBlue kits provide accurate quantification in cuvette or microplate. In qPCR, the dye EvaGreen stands out for its low inhibitory effect and clean HRM curves, favouring reproducibility across instruments.
• Cell biology and imaging: For structural and viability labelling, Biotium offers NucView (activation upon caspase-3 cleavage), the organelle probes MitoView and LysoView, as well as the membrane dyes ViaFluor. TrueBlack quenches tissue autofluorescence, while EverBrite mounting media preserve signal intensity and stability.
• Antibody and protein labelling: The CF Dyes span a broad spectrum (UV to near-IR) with excellent quantum yields and high photostability. Mix-n-Stain kits label antibodies and proteins in minutes, with no purification, for microscopy, flow cytometry and multiplex immunofluorescence.
• Microbiology and vPCR: The reagent PMAxx improves live/dead discrimination prior to PCR by blocking amplification of DNA from compromised cells. Dyes such as BactoView enable rapid analysis of bacterial populations.
• Electrophoresis and proteins: Staining solutions like One-Step Blue and One-Step Lumitein deliver rapid, sensitive visualisation and are compatible with digital documentation.
Why Biotium? For the rare combination of safety (alternatives to EtBr, reduced hazardous waste), sensitivity (low limits of detection), stability (photostability, minimal fading) and simplicity (straightforward protocols, broad compatibility). Agile R&D, an ISO 9001:2015 quality system and meticulous documentation ensure reliable results in R&D, QC and pilot-scale production labs.

Biotium products supplied by Dutscher

Frequently asked questions about the Biotium brand

Where are Biotium reagents designed and manufactured?
Most dyes and kits are developed and made in the United States (Fremont, CA) under an ISO 9001:2015 quality system.

Do the GelRed/GelGreen dyes safely replace ethidium bromide?
Yes — they are non-mutagenic (Ames-type tests), highly sensitive and compatible with blue-light illumination, reducing UV use and hazardous waste.

EvaGreen — is it compatible with HRM and common qPCR instruments?
Designed for qPCR and HRM, it shows low inhibition and works with most platforms and standard master mixes.

How can you quickly label an antibody with CF Dyes?
Mix-n-Stain kits achieve labelling in ~15 min, with no purification, for IF, flow cytometry and multicolour imaging.

What is PMAxx used for in microbiology?
It prevents amplification of DNA from dead cells, optimising vPCR to estimate the viable fraction in samples.

What CSR commitments does Biotium have?
Substitution of EtBr, limiting UV exposure, clear SDS documentation, and processes aimed at reducing the hazard of laboratory waste.