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Laboratory electrophoresis systems: how can DNA, RNA or proteins be separated reproducibly?

Whether you are characterising a protein extract, genotyping an isolate or validating a purification, electrophoresis remains a universal laboratory standard. Our electrophoresis systems cover separation of DNA/RNA on agarose gel, proteins by SDS-PAGE, IEF and Western blot transfers. Designed for precision and safety, they combine horizontal chambers and vertical ones, accessories for casting, combs and trays, plus a programmable power supply. Goal: reproducibility, clear bands and day-to-day productivity.

Which electrophoresis systems are available, and how do you choose the right one?

For DNA/RNA, horizontal systems run easy-to-cast agarose gels using trays and combs that define the number and width of wells. They prioritise a low buffer volume, secure lids and leak-proof casting guides for fast, clean set-up.

For proteins, 1D vertical systems (SDS-PAGE or native) use glass plates and casting chambers that stay leak-tight during polymerisation. Cooling options maintain band resolution and reproducibility. Gradient gels broaden the separable mass range.

In 2D/IEF, the first dimension focuses proteins by pI on a pH gradient (IPG strips), the second separates by mass in PAGE. Precision IEF cells control temperature, voltage ramps and field homogeneity.

Transfer systems (wet tank or semi-dry) move proteins onto PVDF/nitrocellulose membranes for Western blots: semi-dry is fast and buffer-efficient, wet tank excels with large complexes.

The power supply delivers constant voltage/current, programmable profiles, timing and safety features (lid, load detection). Gel dryers facilitate archiving and autoradiography.

Selection criteria: gel format, sample capacity, field uniformity, heat dissipation, buffer volume, compatibility with precast gels, casting ergonomics, safety, traceability and budget.

TypePrincipleApplications/Advantages
Horizontal agaroseDNA/RNA migration in agaroseGenotyping, PCR check, low cost, rapid set-up
Vertical 1D (PAGE)SDS-PAGE/native between platesProtein profiling, purity assessment, high resolving power
2D + IEFpI then mass (IEF ? PAGE)Deep proteomics, isoform resolution, precise pI

In which fields is an electrophoresis system used?

• Life sciences : insert verification, CRISPR, library QC, SDS-PAGE profiles, Western blot.
• Pharmaceutical/biotech : purification monitoring, aggregates, isoforms, antibody IEF, batch release.
• Agri-food : DNA traceability, GMO detection, species authentication, contaminant monitoring.
• Environment : eDNA, microbial fingerprinting, biodiversity monitoring.
• Cosmetics : protein/enzyme QC, raw-material purity verification.
• Chemistry/quality control : characterisation of charged mixtures, synthesis monitoring, training.
In teaching, low-volume horizontal gel tanks offer an excellent balance between hands-on learning and safety.

Available electrophoresis systems and accessories

Frequently asked questions about electrophoresis systems

Horizontal or vertical – which to choose?
: Horizontal for DNA/RNA in agarose (speed, cost). Vertical for proteins (SDS-PAGE) with higher resolution.

Which power supply is suitable?
: Favour constant-voltage/current modes, programmable stepwise ramps, a timer and lid-safety interlocks. The power rating should cover your tanks/chambers and gels.

Does IEF need temperature control?
: Yes – a stable temperature and precise voltage ramps improve focusing, reproducibility and sample protection.

Semi-dry or wet/tank transfer?
: Semi-dry = fast and low buffer use. Wet tank = more universal, effective for large proteins or thick gels.

How do you maintain tanks and trays?
: Rinse with distilled water, avoid solvents/harsh agents, and dry without abrasion. Check seals and leads to preserve integrity and safety.

Do combs affect resolution?
: Yes. Thickness and tooth count set the per-well volume and band sharpness. Match to throughput and the sensitivity required.

Options to cut the environmental footprint?
: Choose low-volume tanks, optimised programmes and durable consumables. Manage buffers and acrylamide via the appropriate waste streams.