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DNA & RNA storage cards: how can genetic material be collected, preserved and transported?

Designed for field collection and long-term archiving, DNA and RNA storage cards capture genetic information within a treated cellulose matrix. Built-in chaotropic and chelating agents lyse, inactivate nucleases and stabilise the sample, enabling ambient-temperature transport with no cold chain. From sampling (capillary blood, buccal cells, swab, crushed insect) to PCR, genotyping or sequencing, a simple punched-out disc is enough. Traceability, reduced logistics costs and biological safety: a proven, efficient and sustainable format.

What types of DNA/RNA collection cards are available, and how do they work?

Storage cards are built on a cellulose matrix impregnated with reagents (chaotropic agents, detergents, chelators) that lyse cells, denature proteins and inactivate RNases/DNases. DNA—and, on dedicated formats, RNA—binds within the fibre and remains stable once dry under controlled storage conditions.
• “Classic” FTA cards: capture and preserve DNA; the punched disc is directly usable in PCR after wash steps.
• Elution cards: formulations that facilitate nucleic-acid release into a buffer for sensitive workflows (qPCR, NGS).
• RNA-dedicated cards: additives enhancing RNase inhibition for expression profiling (RT-qPCR), with tight humidity control.
• Multi-barrier solutions and sachets with desiccant: moisture protection for prolonged storage.
• Accessories: sterile punch tools (1–6 mm discs), templates, card holders, write-on areas and barcodes for traceability.

Key selection criteria: sample type (blood, saliva, swab, tissues, insects), target (DNA vs RNA), duration and conditions of ambient-temperature storage, protocol compatibility (punch-in PCR, extraction, sequencing), and traceability and biosafety requirements. Outcome: robust sampling, fewer cold-chain consumables and streamlined logistics for the biobank, fieldwork and routine use.















TypePrincipleApplications
DNA card (classic FTA)Treated cellulose; DNA lysis and binding; punch usable after wash stepsGenotyping, PCR, forensics, field biobanking
Elution / RNA-dedicated cardFormulation promoting elution; RNase-blocking additivesRT-qPCR, NGS on labile targets, targeted transcriptomics
Cards + multi-barrier protectionSleeve/barrier + desiccant; humidity controlLong-distance transport, archiving at ambient temperature

In which application areas are DNA/RNA storage cards used?

• Life sciences : biobanking, colony genotyping, model organism DNA banks, gene expression monitoring (RT-qPCR on RNA-specific cards).
• Forensic & security : scene-of-crime sampling, DNA profiling, traceable chain of custody.
• Public health & epidemiology : population-level surveillance, DBS in resource-limited settings, sero-genetic studies.
• Veterinary & agri : field screening, pathogen typing, varietal selection.
• Environment : species biomonitoring, swab collection of environmental DNA (per protocol).
• Pharma/CRO : decentralised clinical trials, cold-chain-free logistics, reduced carbon footprint.
• Education : PCR and DNA barcoding practicals made simple and safe.

Available types of DNA/RNA storage cards

Frequently asked questions about DNA/RNA storage cards

How do I choose between a DNA card and an RNA-dedicated card?
Start from the target molecule. For DNA, a standard collection card is sufficient. For RNA, choose an RNase-inhibiting/RNase-free formulation and keep moisture strictly under control.

Can I run PCR directly from a punched disc?
Yes, with many cards after appropriate wash steps. Otherwise perform an elution/extraction to avoid potential inhibitors and maximise sensitivity.

What is the shelf life at room temperature?
DNA is generally stable long term in a dry atmosphere. RNA is more labile (weeks to months) and requires dedicated RNA cards and desiccation.

Do the cards inactivate pathogens?
The embedded reagents markedly reduce the infectivity of many pathogens, but always apply standard precautions and follow your biosafety procedures.

What are best practices for drying and storage?
Air-dry in a low-humidity environment, then seal in a moisture-barrier pouch with desiccant. Avoid humidity and heat; check the humidity indicator regularly if provided.

What sample volume should I apply to the card?
A few microlitres are sufficient; wet the designated collection area evenly without overloading. Refer to the card’s protocol for optimal saturation.

How can I minimise cross-contamination during punching?
Use disposable punch tips or decontaminate between samples (e.g. sodium hypochlorite/ethanol), change the work surface and operate in a clean area.

Can these cards be shipped without UN3373 declaration?
The dry format facilitates shipment without a cold chain. However, always verify IATA/ADR rules and the sample’s classification before dispatch.