14 MOLECULAR BIOLOGY Hot Disco Taq DNA polymerase Hot Disco Taq 5U/µl, with or without buffer and MgCl2 Hot Disco Taq master mix 2X, ready to use z Inactivated at room temperature, activation at 95°C for 15 min z Possible incorporation of dUTP z High specificity and sensitivity for high performance z For low-level target detection, screening, mutliplexing, direct colony PCR, and qPCR applications z Hot Disco Taq polymerase 5 U/µl, with or without 10X buffer and MgCl2 z Complete with dNTPs, the matrix, primers, water, 10X buffer and MgCl2 if not supplied z Several stamps available z Master mix ready to use, addition of matrix, primers and water only z The 2X master mix contains Hot Disco Taq DNA polymerase, dNTPs, MgCl2 at a final concentration of 1.5 mM, and buffer 1 (KCI/(NH4)2 SO4 Tris) or buffer 2 ((NH4)2 SO4) Specificity Optimisation of MgCl2 concentration and required hybridisation temperature - Standard buffer (Tris-HCl pH 8.5, KCl, 15 mM MgCl2, 1% Tween 20) +++ Yes - Ammonium buffer (Tris-HCl pH 8.5, (NH4)2S04, 15 mM MgCl2, 1% Tween 20) + No - Combination buffer (Tris-HCl, pH 8.7, KCl, (NH4)2SO4, 15 mM MgCl2, 1% Tween 20) ++ Depending on the matrix and primers Designation Number of units / Number of 50 µl reactions Without buffer and without MgCl2 € 10X standard buffer and 25 mM MgCl2 € 10X ammonium buffer and 25 mM MgCl2 € 10X combined buffer and mM MgCl2 € Part no. Part no. Part no. Part no. Hot Disco Taq 5U/µl 500 257674 NC - - - 257670 NC - 257782 NC - 1000 257658 NC - 257661 NC - 257659 NC - 257660 NC - 2500 257662 NC - 257665 NC - 257663 NC - 257664 NC - 5000 257666 NC - 257669 NC - 257667 NC - 257668 NC - Designation Number of 50 µl reactions Number of 20 µl reactions Buffer 1 € Buffer 2 € Part no. Part no. Hot Disco Taq master mix 2X 100 250 257683 NC - 257695 NC - 500 1250 257685 NC - 257697 NC - 2500 6250 257684 NC - 257696 NC - 5000 12500 257686 NC - 257698 NC - Hot Disco Taq master mix 2X, Ready to load 100 250 257687 NC - 257691 NC - 500 1250 257689 NC - 257693 NC - 2500 6250 257688 NC - 257692 NC - 5000 12500 257690 NC - 257694 NC - Buffers available separately on page 257 Available in a "Ready to load" version, containing a loading buffer and a red dye to monitor migration: + Can be deposited directly after PCR, on agarose or SDS gel + No interference with PCR, the products obtained can be used for downstream applications (sequencing, etc.) after purification on a column such as PureIT Exo PCR ClearUp Samples available, contact us Hot Disco Taq GC master mix 2X, ready to use z Master mix ready to use, addition of matrix, primers and water only z For the amplification of GC-rich DNA sequences z Minimises the risk of artefacts due to possible aspecific polymerisation before the first denaturation step z The 2X master mix contains Hot Disco Taq DNA polymerase, optimised buffer, dNTPs, 3.0 mM MgCl2 and stabilising agents Designation Number of 50 µl reactions Number of 20 µl reactions Master mix 1 € Master mix 2 € Part no. Part no. Hot Disco Taq 5U/µl 100 250 257675 NC - 257679 NC - 500 1250 257677 NC - 257681 NC - 2500 6250 257676 NC - 257680 NC - 5000 12500 257678 NC - 257682 NC - Available with master mix I or II. : + Master mix I is suitable in most cases. If, however, the results obtained are not satisfactory, we recommend using master mix 2 + Contact us for samples to select the master mix that will optimise your results
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