44 MEDIA AND CHEMICAL PRODUCTS Mac Conkey (agar) - according to pharmacopoeia Differential medium to help identify lactose-fermenting and non-lactosefermenting Enterobacteriaceae. It is recommended for the detection of escherichia coli in water, food, dai ry products and pharmaceutical preparations. Ready-to-use media Enterobacter aerogenes Escherichia coli Part no. Designation Cond. € 693369 100 ml bottle of Mac Conkey broth *- ClearLine® 10 NC - 693448 100 ml bottle of Mac Conkey broth *- ClearLine® 6 NC - z Storage: 2 - 25 °C (dehydrated base and ready-to-use medium) z Incubation z According to ISO 21567: 37 ± 1 °C for 20 - 24 h z According to ISO 21150: 30 - 35 °C for 24 h * Not in accordance with the pharmacopoeia Ready-to-use media Malt (Agar) Recommended for the detection, isolation and enumeration of yeasts and moulds. z Storage: 2 - 25°C (dehydrated base) - 2 - 8°C (ready-to-use medium) z Incubation: 25 - 30 °C for 18 - 72 h Part no. Designation Cond. € 693437 Malt extract agar slant tube - ClearLine® 100 NC - 693370 100 ml bottle of malt extract agar - ClearLine® 10 NC - Ready-to-use media Mannitol mobility nitrate For the presumptive identification of Enterobacteriaceae based on mannitol fermentation, germ mobility and nitrate to nitrite reduction. z Storage: 2 - 25°C (dehydrated base) - 2 - 8°C (ready-to-use medium) z Incubation: 35 ± 2 °C for 18 - 24 h Part no. Designation Cond. € 693461 10 ml mannitol nitrate mobility tube - ClearLine® 6 NC - 693412 10 ml mannitol nitrate mobility tube - ClearLine® 100 NC - Ready-to-use media MRS deMan,Rogosa, Sharpe (Broth) For lactobacillus culture. Part no. Designation Cond. € 693432 9 ml tube of MRS broth - ClearLine® 100 NC - 693344 100 ml bottle of MRS broth - ClearLine® 10 NC - z Storage: 2 - 25 °C (for dehydrated base) and 2 - 8 °C (for ready-to-use media) z Incubation: 35 °C for 3 days or 30 °C for 5 days in a CO2enriched atmosphere Ready-to-use media Mossel (Broth) - EE (Broth) Enrichment broth for Enterobacteriaceae according to Mossel is a selective medium recommended for the investigation of Enterobacteriaceae in foods. Complies with the European Pharmacopoeia. z Storage: 2 - 30°C (dehydrated base) - 2 - 8°C (ready-to-use medium) z Incubation: 37 °C for 24 - 48 h Part no. Designation Cond. € 693413 9 ml tube of Mossel* broth - ClearLine® 100 NC - 693371 100 ml bottle of Mossel broth - ClearLine® 10 NC - * Not in accordance with the pharmacopoeia Universal neutralizer The neutralizer mixture is added to the pre-enrichment medium whenever the presence of disinfectant solution is known or suspected. As a general rule, this mixture should be used at a rate of 10% of the final volume of the pre-enrichment medium. z Storage: 15 - 25 °C in the dark Formula in g/l Disodium phosphate 75.45 Tween 20 20 Sodium thiosulphate 7.8 Tween 80 30 Lecithin 3 Distilled water 950 L-Histidine HCL 1 Part no. Designation Units/box €/ box 693329 Universal neutralizer ClearLine® - 100 ml bottle 10 NC - Ready-to-use media The pH 5.7 medium allows the enumeration of mesophilic lactic acid bacteria Part no. Designation Cond. € 693372 100 ml bottle of MRS agar pH 5.7- ClearLine® 10 NC - MRS deMan,Rogosa, Sharpe pH 5.7 (Agar) z Storage: 2 - 8 °C (for dehydrated base and for ready-to-use media) z Incubation: 35 °C for 3 days or 30 °C for 5 days in a CO2enriched atmosphere Mueller Hinton (Agar) Reference medium for testing the sensitivity of germs to antibiotics and sulphonamides. Ready-to-use media Part no. Designation Cond. € 693373 100 ml bottle of Mueller Hinton agar - ClearLine® 10 NC - z Storage: 2 - 25 °C (for dehydrated base) and 2 - 8 °C (for ready-to-use media) z Incubation: 35 ± 2 °C for 24 h The neutralizer mixture is added to the pre-enrichment medium whenever the presence of disinfectant solution is known or suspected. As a general rule, this mixture should be used at a rate of 10% of the final volume of the pre-enrichment medium. Neutralizer z Storage: 15 - 25 °C in the dark Formula in g/l Disodium phosphate 100.8 L-Histidine HCL 1 Sodium thiosulphate 7.8 Tween 80 30 Lecithin 3 Distilled water 857.4 Part no. Designation Units/box €/ box 693422 Neutralizer ClearLine® - 25 ml tube 100 NC -
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