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Molecular Biology

Molecular biology studies living systems at the level of DNA, RNA and proteins. It relies on integrated workflows ranging from sample preparation to gene and protein expression analysis, including extraction, amplification, quantification and detection, to generate reliable and reproducible data.


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Consumables and small equipment-Single-use products and reusable itemsAutoradiographyBlade and slideBoxCapCaps stripDNA and RNA storage cardFilm, matsFiltrationHomogenisation and grindingHybridizationMagnetic holderMembrane, blotting paper, and radiographic filmPlatePlate and comb for purification automatonRackReagent reservoirSwabTank - cuvetteTrayTubeUltrafiltration and protein concentrationEquipment-Heavy and specialised equipmentCentrifugeCrosslinkerDoc gel, transilluminatorElectrophoresis systemElectroporatorHeat sealerHomogeniser grinderNucleic acid purification automatonPumpShakerSuction systemThermal cyclerWater purificationMeasuring instrument-Appliances and devices for quantifying, controlling or verifying physical, chemical or biological parametersElectrode and probepH meterSpectrophotometryHealth and safety-Personal and collective protection, cleanroomsEye protectionLabellingPipetting-Instruments and consumables for handling liquidsAutomatic dispenserTipBioreactive-Biological compounds used in cultures, dosages or biochemical reactionsCell cultureReagent for molecular biologyChemistry-Reagents, standards and chemical solutions for analyses and preparations.General chemistrySpectroscopy reagentStandardWater analysis

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Molecular biology makes it possible to explore living organisms at the level of nucleic acids (DNA, RNA) and proteins. It represents a central pillar of fundamental research and of industrial, pharmaceutical, agri-food and cosmetic applications. Its objective is to study, amplify and characterize DNA and RNA, analyze gene and protein expression, and gain a better understanding of fundamental biological mechanisms.

Applications in molecular biology rely on complete workflows, from sample preparation to analysis and interpretation of results. These critical steps require precision, reproducibility and technical expertise. Sample preparation includes the grinding, homogenization and lysis of cells, animal or plant tissues and microorganisms. An efficient and reproducible lysis is an essential prerequisite prior to any DNA/RNA extraction, ensuring the reliability of downstream analyses.

The extraction, purification and concentration of nucleic acids can be performed using manual or automated systems, based on columns, magnetic beads, membranes or filtration devices. These steps make it possible to obtain high-quality nucleic acids, compatible with major molecular biology applications: PCR, RT-PCR, qPCR, RT-qPCR and sample preparation for sequencing.

The quantification and quality control of DNA and RNA are key steps in the workflow. They rely on spectrophotometry and fluorimetry to validate extract quality before amplification, quantification or NGS library preparation. The amplification and quantification steps are carried out using PCR and qPCR thermocyclers, while gel electrophoresis, detection and imaging enable the visualization, validation and analysis of amplification products.

Beyond genomics, molecular biology also encompasses proteomics, including extraction, purification, electrophoresis, western blot and ELISA, in order to analyze and quantify protein expression.

Thus, molecular biology stands out as a discipline structured around integrated workflows, linking nucleic acid and protein analysis to produce reliable, comparable and reproducible data, serving the understanding of life, molecule by molecule.

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