Standard Routine DNA polymerases -

Biodyne enzymes

• All enzymes produced are stable at ambient temperature
• A specific genetic modification: "Stability TAG" increases polypeptide shelf-life and its tolerance for temperature at a wider scale without compromising the properties of the polypeptide itself
• After being stored at ambient temperature (tested up to +35 °C), the enzyme remains fully active

Advantages
• Easy Pre-PCR preparation, fast and without any particular precautionary measures (no need for ice bath)
• The best quality/price ratio on the market
• Reduced waste and environmental impact (no need for ice shipping, smaller packaging)

Thermo-Start DNA Taq Polymerase

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• Requires an activation step at 95 °C for 15 minutes
• Inactive at room temperature
• Amplifying fragments up to 5 kb
• Contains
• Thermo start (5U/µl) 10 x 50 µl
• Buffer (10X) 10 x x1.5 ml
• MgCl₂ (25 mm) 10 x 1.5 ml

Taq DNA Polymerase Kit (Thermus aquaticus) 5 U/µl with dNTPs

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• Recombinant protein, ultrapure, at an initial concentration of 5 U/µl
• Conserved 5'-'3' exonuclease activity and absence of 3'-'5' exonuclease activity
• Adds a free adenine to the 3' end of the amplicon (monoadenylation)
• Kit with 3 different 10X reaction buffers: without MgCl₂ (A), with MgCl₂ (B), with 2 inert dyes for direct deposit (C)
• Kit supplied with dNTPs (separate tube)

Color Taq DNA Polymerase Kit (1 U/µl) with dNTPs

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• Recombinant protein, ultrapure, at an initial concentration of 1 U/µl
• Contains 2 inert dyes (red and yellow) to facilitate PCR preparation and allow direct deposition on electrophoresis gel and migration monitoring
• Conserved 5'-'3' exonuclease activity and absence of 3'-'5' exonuclease activity
• Adds a free adenine to the 3' end of the amplicon (monoadenylation)
• Kit with 2 different 10X reaction buffers: without MgCl₂ (A) and with MgCl₂ (B)
• Kit supplied with dNTPs (separate tube)

Fastgene Taq DNA Polymerase

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• Highly purified polymerase by 3 different chromatography methods and very active
• Compatible with TA-cloning
• Concentration: 5 U/µl
• Supplied with 2 separate reaction buffers to choose from depending on the type of PCR and a tube of MgCl₂
• Long-term storage at -20 °C
• Available in 500 U and 2000 U sizes

Fastgene Taq READY MIX 2X

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• PCR mix containing Fastgene Taq DNA polymerase (1 U per 50 µl reaction volume), dNTPs (0.2 mM final of each), MgCl₂ (1.5 mM final) and stabilisation factors
• Supplied with two inert dyes to allow direct deposition of PCR products on agarose gel

Fastgene BAC free HS Taq

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• Recombinant Thermus aquaticus DNA polymerase, cloned, produced and purified from eukaryotic cells
• Can be used for screening bacterial genomes without false positive detection
• Compatible with multiplex PCR, RT-PCR and difficult amplifications

Master mix SG qPCR (2X) and Master mix SG qPCR (2X) with ROX solution

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• SYBR Green I dye qPCR mix for use with most real-time thermal cyclers
• Contains Perpetual Taq hot start DNA polymerase, optimised buffer, dNTPs (dTTP partially replaced by dUTP) and SYBR Green I dye
• Uracile N-glycosylase (UNG) heat-labile provided to limit the risk of cross-contamination (optional use)
• Available with ROX dye (separate tube) for standardisation

Master mix Blank qPCR (2X) and Master mix Blank qPCR (2X) with ROX solution

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• Dye qPCR mix for use with most real-time thermal cyclers
• Contains no dye to allow choice of dye and optimisation of its concentration
• Contains Perpetual Taq hot start DNA polymerase, optimised buffer and dNTPs (dTTP partially replaced by dUTP)
• Uracile N-glycosylase (UNG) heat-labile provided to limit the risk of cross-contamination (optional use)
• Available with ROX dye (separate tube) for standardisation

Fastgene 2X IC green universal qPCR kit with ROX

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• Universal qPCR kit with IC Green fluorescent dye development
• Sensitive and robust
• Can be used for DNA quantification, gene detection, melting curve analysis and more
• Supplied with ROX solution in a separate tube for standardisation

Fastgene 2X IC green universal qPCR kit with Fluorescein

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• Universal qPCR kit with IC Green fluorescent dye development
• Sensitive and robust
• Can be used for DNA quantification, gene detection, melting curve analysis and more
• Reference Description € excl. tax h Supplied with fluorescein solution for standardisation

Master mix Probe qPCR (2X) and Master mix Probe qPCR (2X) with ROX solution

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• QPCR mix with hydrolysis probes for use with most real-time thermal cyclers
• Contains Perpetual Taq hot start DNA polymerase and optimised buffer and dNTPs (dTTP partially replaced by dUTP)
• Uracile N-glycosylase (UNG) heat-labile provided to limit the risk of cross-contamination (optional use)
• Available with ROX dye (separate tube) for standardisation

Fastgene 2X probe universal qPCR kit

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• Universal qPCR kit for hydrolysis probes
• Suitable for multiplex qPCR and rapid qPCR protocols due to robust mix chemistry
• For use in DNA quantification, gene detection, SNP genotyping or NGS validation
• Supplied with ROX solution in a separate tube for standardisation
• Can be used on all real time thermal cyclers

Hi-fi Pop DNA polymerase

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• For applications that require high fidelity such as cloning, sequencing, SNP analysis and mutagenesis
• 60 times higher fidelity than conventional Taq polymerase
• Amplification of long, GC-rich DNA sequences
• Complex matrices (very rich in GC, complex secondary structures, very long sequences, etc.) may require the addition of a 5M betaine enhancer (optional)
• High elongation rate: 10 sec/kb
• 3' -' 5' exonuclease activity