• High throughput and reliable amplification for routine applications, screening or high-throughput screening • Amplification jusqu'à 5 kb, compatible avec le TA-cloning et l'incorporation de dUTP • 5' -> 3' exonuclease activity and absence of 3'-> 5' exonuclease activity
• Inactivated at room temperature, activation at 95°C for 15 min • Incorporation possible de dUTP • High specificity and sensitivity for high performance • Pour détection de cibles même en faible quantité, le screening, le mutliplexage, la PCR directe sur colonies, et les applications de qPCR
• All enzymes produced are stable at ambient temperature • A specific genetic modification: "Stability TAG" increases polypeptide shelf-life and its tolerance for temperature at a wider scale without compromising the properties of the polypeptide itself • After being stored at ambient temperature (tested up to +35 °C), the enzyme remains fully active
Advantages • Easy Pre-PCR preparation, fast and without any particular precautionary measures (no need for ice bath) • The best quality/price ratio on the market • Reduced waste and environmental impact (no need for ice shipping, smaller packaging)
• Recombinant protein, ultrapure, at an initial concentration of 5 U/µl • Conserved 5'-'3' exonuclease activity and absence of 3'-'5' exonuclease activity • Adds a free adenine to the 3' end of the amplicon (monoadenylation) • Kit with 3 different 10X reaction buffers: without MgCl2 (A), with MgCl2 (B), with 2 inert dyes for direct deposit (C) • Kit supplied with dNTPs (separate tube)
• Recombinant protein, ultrapure, at an initial concentration of 1 U/µl • Contains 2 inert dyes (red and yellow) to facilitate PCR preparation and allow direct deposition on electrophoresis gel and migration monitoring • Conserved 5'-'3' exonuclease activity and absence of 3'-'5' exonuclease activity • Adds a free adenine to the 3' end of the amplicon (monoadenylation) • Kit with 2 different 10X reaction buffers: without MgCl2 (A) and with MgCl2 (B) • Kit supplied with dNTPs (separate tube)
• Highly purified polymerase by 3 different chromatography methods and very active • Compatible with TA-cloning • Concentration: 5 U/µl • Supplied with 2 separate reaction buffers to choose from depending on the type of PCR and a tube of MgCl2 • Long-term storage at -20 °C • Available in 500 U and 2000 U sizes