• Reaction mixtures for RT-qPCR in one step • Contains all components necessary for cDNA synthesis and probed qPCR (besides matrix, primers and probes) in a single tube • Probe Kit optimized for singleplex and duplex (1 to 2 targets) • Multiplex Probe Kit Optimized for Multiplex (up to 4 targets) • Optimized for DNA and LNA probes • Probe Kit contains ROX dye for standardization