Molecular biology - Proteins Chromatography By affinity

By affinity

HisTrap™ columns: purification of histidine-tagged proteins

HisTrap HP (High Performance): for purification of histidine-tagged protein after sample filtration. Particle size: 34 µm.

HisTrap FF Crude: for purification of histidine-tagged proteins. No need for filtration of the samples.

HisTrap FF (Fast Flow): for purification of histidine-tagged recombinant proteins from large volume of filtered sample. Particle size: 90 µm.

HiTrap™ columns

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• HiTrap columns are available in 1 ml and 5 ml bed volume
• Each HiTrap™ column is equiped with one 1/16" male luer and one 1/16" female luer. It is possible to connect columns to each other for achieving columns of variable volume from 1 to 20 ml as required
• Concentration or desalting samples
• Purification of recombinant proteins
• Purification of immunoglobulins

Acronyms and abbreviations: Q = Quaternary ammonium / DEAE = Diethylaminoethyle / CM = Carboxymethyle / S = Methylsulfonate / ANX = Diethylaminopropyle

HiTrap Capto™: designed to meet industrial requirements for the production of biological molecules.

HiTrap NHS: high-performance medium designed for easy coupling of ligands containing primary amines.

HiTrap Protein A and HiTrap Protein G: designed for preparative purification of monoclonal and polyclonal antibodies from ascites, serum, and cell culture supernatants.

HiTrap MabSelect™, MabSelect SuRe™ and MabSelect Xtra™: developed to meet the productivity and sanitisation requirements of industry to develop large-scale antibody purification procedures.

HiTrap IgM: designed for purification of monoclonal IgM.

HiTrap IgY: designed for purification of IgY.

HiTrap IXSelect: pour la purification du facteur de coagulation IX.