Molecular biology Specialty ranges of molecular biology reagents Solis BioDyne (PCR-qPCR) SOLIScript One-Step RT-qPCR Probe Kit and Multiplex Probe Kit

SOLIScript One-Step RT-qPCR Probe Kit and Multiplex Probe Kit

• Reaction mixtures for RT-qPCR in one step
• Contains all components necessary for cDNA synthesis and probed qPCR (besides matrix, primers and probes) in a single tube
• Probe Kit optimized for singleplex and duplex (1 to 2 targets)
• Multiplex Probe Kit Optimized for Multiplex (up to 4 targets)
• Optimized for DNA and LNA probes
• Probe Kit contains ROX dye for standardization

Variants and accessories

One-Step RT-qPCR

Multiplex One-Step RT-qPCR

Probe Kit consisting of three reagents• Mix qPCR 5x with DNA polymerase HOT FIREPol, reaction buffer, dNTPs (without dUTP), 15 mm MgCl₂ (final concentration 3 mm), ROX dye
• Mix SOLIScript 40x with SOLIScript reverse transcriptase and RIBOGRIP ribonuclease inhibitor
• Molecular biology quality Water

Multiplex Probe Kit consisting of three reagents• qPCR 5x Mix with HOT FIREPol DNA polymerase, reaction buffer, dNTPs (dUTP included), 15 mm MgCl₂ (final concentration 3 mm)
• SOLIScript 40x Mix with SOLIScript reverse transcriptase and RIBOGRIP ribonuclease inhibitor
• Molecular biology quality Water
• Mix also available with ROX or PURPLE dye for thermocyclers requiring a reference signal for data normalization

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Specific terms
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