258507 · Master Mix PCR Owi Taq Hot Start Ready to Load, 20 reactions of 50 µl

• For the detection of low-abundance targets, screening, multiplexing, and direct PCR on colonies
• Enzyme inactivated at room temperature, activated at 95°C for 15 minutes
• Possible incorporation of dUTP
• High specificity and sensitivity, for high yield even with GC- or AT-rich sequences
• Amplification possible up to 5 kb
• Ready-to-use master mix: only the template, primers, and water need to be added
• Dye included for migration tracking and direct deposition on agarose or SDS gel after PCR
• Contains: Owi Taq HS polymerase, dNTPs (0.4 mM), MgCl₂ (final concentration 1.5 mM), Tris-HCl buffer pH 8.5, (NH₄)₂SO₄, 3.0 mM MgCl₂, 0.2% Tween 20, inert blue dye and stabiliser