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• Reaction mixtures for one-step RT-qPCR • CDNA synthesis and qPCR for up to 5 targets in around one hour • Ready-to-use kit in a single tube containing : SOLIScript reverse transcriptase, Ribogrip RNAse inhibitor, Salini UNG™ enzyme, 5X qPCR mix (HOT FIREpol polymerase, nucleotides, MgCl₂ (12.5 mM), ROX) and molecular biology water • Addition of matrix, primers and probes only • Salini UNG™ uracil-N-Glycosylase reduces the risk of cross-contamination by removing DNA from previous PCR reactions