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Molecular Biology - Nucleic Acids PCR in end point Hot Start DNA polymerases

Hot Start DNA polymerases

Biodyne HOT FIREPol®

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tiAmplus DNA Polymerase

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• Optimized mixture of thermostable DNA polymerases Taq (Thermus aquaticus), Pfu (Pyrococcus furiosus) and a polymerization activator
• Ultrapure recombinant polymerases, thermostable and hot start. Pfu proofreading
• The hot start property of the polymerases makes them inactive at room temperature allowing the preparation of the reaction mix at room temperature
• Unique composition of the reaction buffer promoting pH stability at high temperature and ensuring high efficiency for long sequence amplifications
• Amplification of genomic DNA sequences from 3 to 25 kb and episomal DNA sequences up to 40 kb
• Particularly suitable for the amplification of eukaryotic genomes
• Ideal for genome mapping and sequencing of long fragments
• Supplied with a 10X reaction buffer
• Available in 100 and 500 unit formats

SolisFAST ADN Polymerase

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• For amplification in fast PCR mode, reduces the time of a PCR cycle by up to 4 times compared to a standard protocol.
• Elongation step 3 to 4 times faster than with conventional DNA polymerase
• Hot start enzyme inactivated at room temperature, activation in only 30 seconds at 98 °C
• For the amplification of Low complexity DNA fragments (cDNA, lambda, plasmid DNA) up to 5 kb or up to 3 kb for human, animal or plant DNA
• Ready-to-use mix Kit; requires only the matrix, primers and molecular biology quality water
• Available in a 'Ready to load' version for direct deposit on gel, contains a load buffer and two dyes for migration monitoring (blue (3,5-4,5 kb) and yellow (35-45 pb))
• Available in a version with UNG to reduce the risk of cross-contamination
• Stable 30 days at room temperature, long-term storage at -20 °C

qPCR SolisFAST Mix

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• Mix for quantitative PCR with fast protocol, elongation 3 to 4 times faster than with conventional DNA polymerase
• Hot start enzyme inactivated at room temperature, activation in only 30 seconds at 95 °C
• qPCR results obtained in only 30 minutes depending on thermocycler performance (optimized for qPCR Mic thermocyclers (Cat No. 634001 and 634002)
• Mix also available with ROX or PURPLE dye for thermocyclers requiring a reference signal for data normalization.
• Stable 30 days at room temperature, long-term storage at -20 °C

PAXgene Blood sampling Tubes

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• For the stabilization of DNA, RNA or circulating, cell-free DNA (ccfDNA) depending on Cat.No.
• Secure and standardized sampling
• CE/IVD certified
• Compatible with sensitive applications: RT-qPCR, NGS, methylation...

OnTaq DNA Polymerase Hot Start Kit (2.5 U/µl) with dNTPs

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• DNA polymerase hot start at an initial concentration of 2.5 U/µl
• Increases PCR specificity, sensitivity and throughput by reducing the risk of mismatch and primer-dimer formation
• Activated during the initial 10 min denaturation step at 95 °C
• Conserved 5'-'3' exonuclease activity and absence of 3'-'5' exonuclease activity
• Adds a free adenine to the 3' end of the amplicon (monoadenylation)
• Kit with 3 different 10X reaction buffers: without MgCl2 (A), with MgCl2 (B), with 2 inert dyes for direct deposit (C)
• Kit supplied with dNTPs (separate tube)

Fastgene ApTaq Hot Start DNA polymerase

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• DNA polymerase Taq hot start
• Hot start system by aptamer technology: reversible activation / inactivation
• Polymerase inactivated below 45 °C
• Fully activated polymerase above 60 °C
• No specific activation step required as with antibody technology
• Ideal for rapid PCR, routine PCR and SNP analysis in particular

Master mix Fast Sybr Green qPCR (2X) and Master mix Fast SG qPCR (2X) with ROX solution

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• Fast qPCR mix with SYBR Green I dye for use with most real-time thermal cyclers
• Contains Perpetual Taq hot start DNA polymerase, optimised buffer, dNTPs (dTTP partially replaced by dUTP) and SYBR Green I dye
• Uracile N-glycosylase (UNG) heat-labile provided to limit the risk of cross-contamination (optional use)
• Available with ROX dye (separate tube) for standardisation

Master mix Fast Probe qPCR (2X) and Master mix Fast Probe qPCR (2X) with ROX solution

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• Fast qPCR mix with hydrolysis probes for use with most real-time thermal cyclers
• Contains Perpetual Taq hot start DNA polymerase, optimised buffer, dNTPs (dTTP partially replaced by dUTP) and SYBR Green I dye
• Uracile N-glycosylase (UNG) heat-labile provided to limit the risk of cross-contamination (optional use)
• Available with ROX dye (separate tube) for standardisation

Master mix HRM PCR (2X)

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• Mix designed for high resolution melting curve (HRM) analysis of DNA samples
• Allows detection of gene mutations and single polymorphisms (SNPs) including class III and IV
• Contains onTaq hot start DNA polymerase, optimised buffer, dNTPs and fluorescent dye
• OnTaq polymerase activated during the initial denaturation step of the PCR at 95 °C for 15 min
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