cDNA - RT-PCR synthesis kits -

OneStep RT-PCR Kit

• RT-PCR single step reaction
• Master mix containing a highly processive dART reverse transciptase and a "hot start" DNA polymerase and the high-temperature active RNase inhibitor
• 2X reaction buffer with dNTPs, stabilizers and reaction enhancements

AMV Reverse Transcriptase Native (Avian Myeloblastosis Virus)

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• Native reverse transcriptase with a
• Retained RNase H
• Wide range of operating temperatures between 37 and 65 °C
• Ideal for RT-PCR of GC-rich templates with a high degree of secondary structures, cDNA library synthesis and Sanger sequencing in particular

SmART RT-PCR Kit

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• SmART reverse transcriptase-based two-step RT-PCR kit
• Reduced RNase H activity and increased thermostability and processivity of RT between 37 and 65 °C
• Second strand synthesis (PCR) performed with OptiTaq high fidelity DNA polymerase
• Ideal for cloning and diagnostics on fragments up to 7 kb

RT-PCR Kit NG dART

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• NG dART reverse transcriptase-based two-step RT-PCR kit
• Improved thermostability (up to 65 °C) and increased processability of RT
• The PCR step is performed in a separate tube with the high fidelity OptiTaq
• RT in master mix format for easy use of the kit and to reduce pipetting errors

One Step RT-PCR Kit

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• One-step RT-PCR kit based on thermostable reverse transcriptase and hot start Taq polymerase
• Blend RT-RI 20X with reverse transcriptase and RNase inhibitor for high yields
• OneStep 2X mix with Taq hot start, dNTPs, MgCl₂ and additives in a buffer optimised for low background and high sensitivity
• For complex matrices, rich in GC or AT, and/or in low abundance in the starting sample

SmART RT-qPCR Kit and smART RT-qPCR Kit plus ROX Solution

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• SmART reverse transcriptase-based two-step real-time RT-PCR kit
• Reduced RNase H activity and increased thermostability and processivity of RT between 37 and 65 °C
• Real-time PCR step performed with Master Mix SG qPCR 2X, qPCR with SYBR Green I probe
• Contains a high-performance hot-start DNA polymerase ideal for real-time diagnostics
• Contains uracil-N-glycosylase (UNG) to prevent cross-contamination
• Available with ROX dye for standardisation

Sybr Green OneStep qRT-PCR and Sybr Green OneStep qRT-PCR plus ROX solution kits

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• One-step real-time RT-PCR kit with SYBR Green I dye
• Enzyme master mix consisting of reverse transcriptase, onTaq's highly processive hot start DNA polymerase and a Rnase inhibitor
• Buffer mix containing dNTPs (dTTP partially replaced by dUTP) and SYBR Green I dye
• Uracile N-glycosylase heat-labile provided to limit the risk of cross-contamination
• Available with ROX solution (separate tube)

Sybr Green OneStep PRO RT-qPCR Kit

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• One-step real-time RT-PCR kit with SYBR Green I dye
• Proofreading activity maintained during retrotranscription and PCR
• SG Pro enzyme master mix consisting of a reverse transcriptase active at 52-72 °C without loss of specificity or sensitivity, a DNA polymerase and an RNase inhibitor
• Buffer mix containing dNTPs (dTTP partially replaced by dUTP) and SYBR Green I dye
• Uracile N-glycosylase (UNG) heat-labile provided to limit the risk of cross-contamination (optional use)

Fastgene IC Green one step RT-qPCR mix with ROX

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• One-step RT-qPCR mix with IC Green fluorescent dye
• Contains reverse transcriptase and DNA polymerase in a buffer optimised to combine enzyme processivity and reaction sensitivity
• Compatible with fast and ultra-fast cycle programs
• Can be used for DNA quantification, gene detection, melting curve analysis and more
• Available in Hi-Rox and Low-Rox versions, for 100 or 500 reactions of 20 µl

Probe OneStep RT-qPCR and OneStep RT-qPCR kits plus ROX solution

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• One-step RT-qPCR kit
• Enzyme master mix containing highly sensitive reverse transcriptase, highly processive onTaq hot start DNA polymerase and Rnase inhibitor 2X buffer containing dNTPs (dTTP partially replaced by dUTP) Heat-labile Uracile N-glycosylase provided to limit cross-contamination Available with ROX solution (separate tube)

Fastgene probe one step RT-qPCR mix with ROX

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• One-step RT-qPCR mix designed to use probes including TaqMan®, Scorpions® and molecular beacon probes
• Contains an efficient reverse transcriptase stable at 45-55 °C and a hot start DNA polymerase
• Can be used for RNA quantification, pathogen detection, high throughput gene screening..
• Compatible with fast cycling protocols
• Available in Hi-Rox and Low-Rox versions, for 100 or 500 reactions of 20 µl

SolisGreen 1-step RT-PCR Kit

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• Reaction mixtures for one-step RT-qPCR
• One-tube cDNA synthesis and qPCR with the SybrGreen fluorescent intercalator (SYBR or FAM channel)
• Ready-to-use kit containing SOLIScript reverse transcriptase, Ribogrip RNAse Inhibitor, 5X qPCR mix (HOT FIREpol polymerase, nucleotides, MGCl₂ (12.5 mM), SolisGreen intercalant, Rox) and molecular biology water
• Addition of matrix and primers only
• Compatible with ROX-dependent and independent thermal cyclers

SOLIScript Fast 1-Step RT-qPCR master mix, ready to use

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• Reaction mixtures for one-step RT-qPCR
• CDNA synthesis and qPCR for up to 5 targets in around one hour
• Ready-to-use kit in a single tube containing : SOLIScript reverse transcriptase, Ribogrip RNAse inhibitor, Salini UNG™ enzyme, 5X qPCR mix (HOT FIREpol polymerase, nucleotides, MgCl₂ (12.5 mM), ROX) and molecular biology water
• Addition of matrix, primers and probes only
• Salini UNG™ uracil-N-Glycosylase reduces the risk of cross-contamination by removing DNA from previous PCR reactions