Molecular Biology - Nucleic Acids PCR in end point Hot Start DNA polymerases SolisFAST ADN Polymerase

SolisFAST ADN Polymerase

• For amplification in fast PCR mode, reduces the time of a PCR cycle by up to 4 times compared to a standard protocol.
• Elongation step 3 to 4 times faster than with conventional DNA polymerase
• Hot start enzyme inactivated at room temperature, activation in only 30 seconds at 98 °C
• For the amplification of Low complexity DNA fragments (cDNA, lambda, plasmid DNA) up to 5 kb or up to 3 kb for human, animal or plant DNA
• Ready-to-use mix Kit; requires only the matrix, primers and molecular biology quality water
• Available in a 'Ready to load' version for direct deposit on gel, contains a load buffer and two dyes for migration monitoring (blue (3,5-4,5 kb) and yellow (35-45 pb))
• Available in a version with UNG to reduce the risk of cross-contamination
• Stable 30 days at room temperature, long-term storage at -20 °C

Variants and accessories

Master Mix SolisFast 5X

Master Mix SolisFast 5X with UNG

• The 5X buffer contains: 17,5 mM de MgCl₂, the dNTPs and additives to ensure maximum efficiency of the PCR reaction

• The 5X buffer contains: 17,5 mM de MgCl₂, dNTPs (dATP, dCTP, dGTP, dUTP), UNG and additives to ensure maximum efficiency of the PCR reaction
• Mix containing the enzyme Uracil-DNA glycosylase (UNG) and dUTPs instead of dTTPs
• Reduces the risk of cross-contamination by removing DNA from the previous PCR reaction:
- the enzyme uracil-DNA glycosylase (UNG) degrades DNA containing uracil residues at the beginning of the reaction, before being inactivated during the activation phase of the DNA polymerase
- dUTPs are incoporated instead of dTTPs during the DNA elongation step

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Specific terms
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