Molecular Biology - Nucleic Acids PCR in end point Hot Start DNA polymerases qPCR SolisFAST Mix

qPCR SolisFAST Mix

• Mix for quantitative PCR with fast protocol, elongation 3 to 4 times faster than with conventional DNA polymerase
• Hot start enzyme inactivated at room temperature, activation in only 30 seconds at 95 °C
• qPCR results obtained in only 30 minutes depending on thermocycler performance (optimized for qPCR Mic thermocyclers (Cat No. 634001 and 634002)
• Mix also available with ROX or PURPLE dye for thermocyclers requiring a reference signal for data normalization.
• Stable 30 days at room temperature, long-term storage at -20 °C

Variants and accessories

SolisGreen 5X

Probe 5X

Probe 5X con UNG

• Mix for qPCR with SolisFast DNA polymerase and SolisGreen fluorescent DNA marker
• Ready to use: addition of matrix, primers and water only
• The 5X buffer contains: 7.5 mM of MgCl₂, dNTPs stabilizers and enhancers that maximize efficiency of PCR reaction

• Ready to use: addition of matrix, primers, probes and water only
• The 5X buffer contains: 15 mM of MgCl₂, dNTPs stabilizers and enhancers that maximize efficiency of PCR reaction
• Possibility of multiplex qPCRs (up to 5 simultaneous targets)

• Ready to use: addition of matrix, primers, probes and water only
• The qPCR 5X buffer contains: 16,5 mM MgCl₂ (1x PCR solution 3,3 mM MgCl₂), dNTPs (dATP, dCTP, dGTP, dUTP) and additives to ensure maximum efficiency of the PCR reaction
• The mix includes Uracil-DNA glycosylase (UNG)enzyme and dUTPs enzyme instead of dTTPs
• Reduces the risk of cross-contamination by eliminating DNA from previous PCR reaction:
- the uracil-DNA glycosylase (UNG) enzyme degrades the DNA containing uracil residues at the beginning of the reaction, before being inactivated during the activation phase of the polymerase
- the dUTPs are incoporated instead of dTTPs during the DNA elongation step
• Possibility of multiplex qPCRs (up to 5 simultaneous targets)

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OnTaq DNA Polymerase Hot Start Kit (2.5 U/µl) with dNTPs

Fastgene ApTaq Hot Start DNA polymerase

Master mix Fast Sybr Green qPCR (2X) and Master mix Fast SG qPCR (2X) with ROX solution

Master mix Fast Probe qPCR (2X) and Master mix Fast Probe qPCR (2X) with ROX solution

Master mix HRM PCR (2X)

Specific terms
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