Molecular Biology - Nucleic Acids PCR in end point Hot Start DNA polymerases OnTaq DNA Polymerase Hot Start Kit (2.5 U/µl) with dNTPs

OnTaq DNA Polymerase Hot Start Kit (2.5 U/µl) with dNTPs

• DNA polymerase hot start at an initial concentration of 2.5 U/µl
• Increases PCR specificity, sensitivity and throughput by reducing the risk of mismatch and primer-dimer formation
• Activated during the initial 10 min denaturation step at 95 °C
• Conserved 5'-'3' exonuclease activity and absence of 3'-'5' exonuclease activity
• Adds a free adenine to the 3' end of the amplicon (monoadenylation)
• Kit with 3 different 10X reaction buffers: without MgCl₂ (A), with MgCl₂ (B), with 2 inert dyes for direct deposit (C)
• Kit supplied with dNTPs (separate tube)

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